mont s obalkou.qxd - 13th International Symposium on Aquatic

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13th International Symposium on Aquatic Oligochaeta
B R N O , C Z E C H R E P U B L I C , 7 - 11 S E P T E M B E R 2 0 1 5
Book of Abstracts
Book of Abstracts
13th International Symposium on Aquatic Oligochaeta
Brno, Czech Republic, 7-11 September 2015
Organized by Department of Botany and Zoology,
Faculty of Science, Masaryk University, Brno
Editor: Jana Schenková
Reference
SCHENKOVÁ
Jana
(Ed.).
13 th
International
Symposium
on
Aquatic
Oligochaeta: Book of Abstracts. Brno, Masaryk University, 2015.
Organizing Committee
Chair:
Jana Schenková, Masaryk University, Brno, CR
Co-chair: Mark J. Wetzel, Illinois Natural History Survey, Champaign,
Illinois, USA
Members: Michal Schenk, Arcus Engineering, Brno, CR
Martina Bílková, Masaryk University, Brno, CR
Michal Horsák, Masaryk University, Brno, CR
Veronika Horsáková, Masaryk University, Brno, CR
Dana Hřívová, Masaryk University, Brno, CR
Petr Pařil, T. G. Masaryk Water Research Institute, CR
Marek Polášek, Masaryk University, Brno, CR
Vendula Polášková, Masaryk University, Brno, CR
Jiří Schlaghamerský, Masaryk University, Brno, CR
Jana Zahrádková, T. G. Masaryk Water Research Institute, CR
Published by
Masaryk University, Žerotínovo nám. 671/9, 601 77 Brno, 1. edition, 2015
ISBN 978-80-210-7910-6 (paperback)
ISBN 978-80-210-7911-3 (online: pdf)
Print
Tiskárna Matula, Olomoucká 27, 618 00 Brno, CR
Note
Abstracts in proceedings were not reviewed; authors are responsible for the
content and formal validity of their contributions.
2
ISAO 2015, 13th International Symposium on Aquatic Oligochaeta, Brno, Czech Republic, 7-11 September 2015
International Scientific Advisory Committee
Chair: Mark J. Wetzel, Illinois Natural History Survey, Champaign, Illinois, USA
Members: Naime Arslan, Department of Biology, Faculty of Arts and Sciences,
Eskisehir Osmangazi University, Eskisehir, Turkey
Christer Erséus, Swedish Museum of Natural History, Stockholm,
Sweden
Patrick Martin, Royal Belgian Institute of Natural Sciences, Bruxelles,
Belgium
Adrian Pinder, Department of Parks and Wildlife, Bentley Delivery
Centre, Western Australia
Pilar Rodriguez, University
Leioa-Bizkaia, Spain
of
Basque
Country
UPV/EHU,
Jana Schenková - Symposium Host and Organizer - Department of
Botany and Zoology, Faculty of Science, Masaryk University, Brno,
Czech Republic
Tarmo Timm, Estonian University of Life Sciences, Centre for
Limnology, Rannu, Tartumaa, Estonia
Piet Verdonschot, DLO Institute for Forestry and Nature Reserch,
Wageningen, the Netherlands
3
4
INDEX
INDEX
Welcome to the symposium................................................................. 7
General program................................................................................. 9
List of Posters..................................................................................... 14
Abstracts............................................................................................ 15
List of Participants.............................................................................. 58
Author's Index.................................................................................... 61
Sponsors............................................................................................ 63
5
6
WELCOME TO THE SYMPOSIUM
WELCOME TO THE SYMPOSIUM 13TH ISAO
Dear oligochaetologists, colleagues and friends: it is with great
pleasure that we welcome you to Brno to participate in the 13th
International Symposium on Aquatic Oligochaeta. The triennial ISAO
meetings have been organized since 1979, convening in Sidney, British
Columbia, Canada (1979), Palanza, Italy (1982), Hamburg, Germany
(1985), Baton Rouge, Louisiana, USA (1988), Tallinn, Estonia (1991),
Strömstad, Sweden (1994), Presque Isle, Maine, USA (1997), Bilbao,
Spain (2000), Wageningen, The Netherlands (2003), Wuhan, China
(2006), Antalya, Turkey (2009), and Fremantle, Western Australia (2012).
Our symposia provide a place for scientific communication and
collaboration in the diverse field of aquatic oligochaete research.
Fortunately, in contrast to the rapid development in oligochaete
taxonomy and phylogeny and other fields, where big changes come every
year, many of the scientists participating in the early ISAO meetings
continue with their study of oligochaetes and other groups of annelids,
thus each symposium is a great opportunity to renew and expand our
friendships with colleagues we met during previous symposia, and as
importantly, to welcome new contemporaries and also the younger,
incoming generation of oligochaete biologists. The ISAO13 organizing
committee, and their colleagues, families, and friends have worked hard
during the past four years to organize this 13th ISAO meeting. You will
have opportunities during this symposium to share your knowledge in
various areas of research on oligochaetes, to discuss problems with
colleagues in both formal and informal atmospheres, and to experience
and enjoy the people, language, culture, cuisine, and geography of the
Czech Republic. We hope that our efforts to integrate this professional
meeting with the beauty of Brno, Moravia, and this country will result in
creative, relaxing, and memorable experiences for you during your stay.
Your Organizing Committee
7
8
GENERAL PROGRAM
GENERAL PROGRAM
6 September, Sunday
15:00-19:00 Registration desk opened - Foyer in front of Hall 2
19:30-21:30 Welcome Drink - Foyer in front of Hall 2
7 September, Monday
8:00-12:00
Registration desk open - Foyer in front of Hall 2
Opening - Hall 2
9:00-9:15
Opening speech and welcome, Jana Schenková
9:15-9:30
Welcome speech, doc. RNDr. Jaromír Leichmann, Dr., the Dean of
the Faculty of Science, Masaryk University
9:30-9:40
Welcome and Acknowledgements, Mark J. Wetzel, General
Secretary, ISAO
9:40-9:50
In Memoriam - Beatrice M. Sambugar (Italy), who passed away
early last year, Enrique Martínez-Ansemil and Patrick Martin,
presented by Mark J. Wetzel
9:50-10:10
History: International Symposia of Aquatic Oligochaetes, 19792015, Mark J. Wetzel
10:10-10:40 Coffee Break
Session 1: SYSTEMATICS AND PHYLOGENY
Chairman: Christer Erséus
10:40-11:20 Christer Erséus (keynote lecture). From DNA barcoding and
species delimitation to transcriptome phylogenies - on ongoing
molecular systematics research on clitellates
11:20-11:40 Irina Kaygorodova. Advantages of DNA-barcoding for
understanding of the species diversity within freshwater annelids
of Eastern Siberia
11:40-12:00 Ainara Achurra. Disentangling the Lumbriculidae: preliminary
results from a multilocus phylogenetic analysis
12:00-12:20 Akifumi Ohtaka. Diversity of Aulodrilus (Clitellata, Tubificinae) in
East and Southeast Asia
12:20-14:00 Lunch - Continental Hotel Restaurant
Chairman: Mark J. Wetzel
14:00-14:20 Svante Martinsson. On the systematics Chamaedrilus and
Euenchytraeus (Enchytraeidae)
9
GENERAL PROGRAM
O
14:20-14:40 Marten Eriksson. Systematics of Lumbricillus (Clitellata,
Enchytraeidae) using molecular and morphological approaches
14:40-15:00 Pierre de Wit. An updated phylogeny of the marine enchytraeid
genus Grania supports cryptic speciation and geographic
structuring
15:00-15:20 Yingkui Liu. Extensive cryptic diversity and taxonomic confusion in
the cosmopolitan Limnodrilus hoffmeisteri (Clitellata, Naididae)
16:15-18:00 Walk around Brno: start in front of Continental Hotel - walk
around Brno historical centre - Saint James Church, Cabbage
Market and Parnassus Fountain, Old City Hall, The Cathedral of
Saints Peter and Paul and Špilberk Castle
Group photo of symposium participants
19:00
Snack to accompany beer at the Brewery Restaurant
8 September, Tuesday
Session 2: COMMUNITY ECOLOGY
Chairman: Patrick Martin
9:00-9:40
Göran Milbrink (keynote lecture). Species specific morphological
deformities in oligochaetes associated with mercury emissions.
Lake Vänern, Southern Sweden, revisited
9:40-10:00
Naime Arslan. The use of BMWP and ASPT indices for evaluation
of water quality according to macroinvertebrates in Küçük
Menderes Stream (Turkey)
10:00-10:20 Steven V. Fend. Annelids in hypereutrophic Upper Klamath Lake,
Oregon, USA
10:50-11:10 Fatemeh Nazarhaghighi. Oligochaetes (Annelida, Clitellata) in
the Anzali International Wetland, Northwestern Iran
11:10-11:30 Jana Schenková. Can oligochaete assemblages predict an
ecological type of treeless spring fens?
11:30-11:50 Jiří Schlaghamerský. Annelids of six calcareous spring fens and
adjacent grasslands in the Western Carpathians
11:50-12:10 Haifa J. Jaweir. Aquatic oligochaetes community in AL-Dalmage
lake/ Iraq
10
GENERAL PROGRAM
Chairman: Pilar Rodriguez
14:00-14:20 Kimio Hirabayashi. Profundal benthic macroinvertebrates in
Japanese lakes of different trophic status
14:20-14:40 Deniz Mercan. Phylogeography and geographic genetic analysis
of Potamothrix hammoniensis (Clitellata) which is a Ponto-Caspian
origin having distribution in some fresh water lakes of Turkey
14:40-15:00 Petr Pařil. The role of Oligochaeta in the bioindication of stream
intermittency - results of the BIODROUGHT project
15:00-15:20 Rüdiger M. Schmelz. Phreodrilidae in Irish peatlands
15:20-15:40 Mohammed Ibrahim Naveed. Preliminary survey of fresh water
Oligochaeta from selected districts of Tamil Nadu
16:00-18:00 Poster Session
19:00
Dinner on your own
9 September, Wednesday
All day excursion to two beautiful places in Moravia, Pernštejn Castle and
Macocha Abyss in the Moravian Karst. Two buses will go to both places but in
opposite order, lunch in the Skalní Mlýn (Rock Mill) Hotel will be meeting point of
both groups.
1. bus departure 7:45 hotel-Pernštejn Castle-lunch-Macocha Abyss-hotel (18:30)
2. bus departure 7:45 hotel-Macocha Abyss-lunch-Pernštejn Castle-hotel (18:30)
19:00
Dinner on your own
10 September, Thursday
Session 3: MORPHOLOGY
Chairman: Steven V. Fend
9:00-9:40
Pilar Rodriguez (keynote lecture). Sperm transfer by
spermatophores in microdrile oligochaetes (Annelida, Clitellata)
9:40-10:00
Piotr Świątek. Structure and evolution of ovaries in oligochaetous
annelids: recent progress and things to do
10:00-10:20 Yu Peng. Aquatic oligochaetes from southern Tibet, China
11
GENERAL PROGRAM
Session 4: ECOTOXICOLOGY
Chairman: Olav Giere
10:50-11:10 Olav Giere. Life in a toxic environment - an exceptional new
"sulphur worm" of the genus Limnodrilus
11:10-11:30 Mana Ito. Marine oligochaete, Thalassodrilides sp.; a potential
candidate for bioremediation of polycyclic aromatic hydrocarbonscontaminated sediment
11:30-11:50 Katsutoshi Ito. Research on the seasonal variation of biomass of
marine oligochaeta Thalassodrilides sp. with high pollution
tolerance in Fukuura Bay, Japan and its efficient culture condition
11:50-12:10 Leire Méndez-Fernández. Metal tissue baseline concentrations in
aquatic oligochaetes from mining areas of Northern Spain
DISCUSSION GROUP
14:00-15:00 Space for informal discussion
MICROSCOPY SESSION
15:30-15:45 Jendrulek Tomáš. Super resolution in confocal microscopy
(presentation of symposium sponsor Olympus)
15:45-17:00 Free work on microscopes and consultations on your own
material
19:00
Dinner on your own
11 September, Friday
Session 5: BIOGEOGRAPHY
chairman: Mark J. Wetzel
9:00-9:40
Tarmo Timm (keynote lecture). Fate of Lamprodrilus isoporus
Michaelsen, 1901 (Oligochaeta: Lumbriculidae) in eutrophic lakes
9:40-10:00
Elzbieta Dumnicka. Alien species from selected annelids families
and their role in aquatic habitats in Poland
10:00-10:20 Irina Kaygorodova. Oligochaete communities in anomalous
regions of Lake Baikal, Eastern Siberia, Russia
10:50-11:10 Mark J. Wetzel. Annelidically Speaking-2015.
12
GENERAL PROGRAM
11:10-11:30 Patrick Martin. The groundwater oligochaetes (Annelida,
Clitellata) from the "Parc du Mercantour" (France)
11:30-11:50 Yongde Cui. Macroecological patterns of macrozoobenthos
in rivers of China
ISAO BUSINESS MEETING
14:00-16:00 Host recognition (next symposium): No host or venue has yet been
determined - so we encourage all ISAO13 participants to consider
hosting ISAO14 (in 2018).
16:30-16:40 Presentation of information on symposium proceedings,
Jana Schenková
16:40-16:50 Post-symposium tour information, Jana Schenková
16:50-17:00 Official closing of the 13th ISAO symposium, Mark J. Wetzel,
Jana Schenková
19:00
Closing Banquet - Hall 2
13
POSTERS
POSTERS
Seval Aras, Özlem Findik
Aquatic Oligochaeta (Annelida) Lakes of Aşagi Firat River Basin................ 16
Martina Bílková, Jana Schenková
Carpathian spring fens versus nearby brooks: what does Trichodrilus strandi
(Lumbriculidae) like more?.......................................................................... 18
Özlem Findik, Seval ARAS
Aquatic Oligochaeta (Annelida) of Ceyhan River Basin's Lakes (TURKEY).... 24
Szymon Gorgoń, Bartosz J. Płachno, Richard Marchant, Mariola Krodkiewska,
Karol Małota, Anna Z. Urbisz, Piotr Świątek
Ovary structure in basal Oligochaeta.......................................................... 26
Mariola Krodkiewska, Maciej Kostecki
The use of biological indices based on bottom oligochaetes in the assessment
of restoration measures in an anthropogenic reservoir (Pławniowice Reservoir,
southern Poland)......................................................................................... 33
Jeounghee Lee, Jongwoo Jung
Diversity of Oligochaeta in Korea................................................................ 34
Enrique Martínez-Ansemil, Federica Giacomazzi, Beatrice Sambugar†
Groundwater oligochaetes (Annelida, Clitellata) of the Dinaric region........37
Anna Z. Urbisz, Łukasz Chajec, Piotr Świątek
Ovary composition and oogenesis in the sludge worm Tubifex tubifex
(Tubificinae)................................................................................................. 55
14
ABSTRACTS
ORAL
ABSTRACTS
Disentangling the Lumbriculidae: preliminary results from a
multilocus phylogenetic analysis
Ainara Achurra (1), Pilar Rodriguez (1), Steven V. Fend (2) & Christer Erséus (3)
(1) Department of Zoology and Animal Cell Biology, Faculty of Science and
Technology, University of the Basque Country, Leioa, Spain; (2) Department of
Biological and Environmental Sciences, University of Gothenburg, Gothenburg,
Sweden; (3) U.S. Geological Survey, Menlo Park, California, USA.
The phylogenetic relationships of selected members of the family
Lumbriculidae are explored using six DNA markers: the nuclear genes 18S rRNA,
28S rRNA and histone H3, and the mitochondrial genes 12S rRNA, 16S rRNA and
cytochrome c oxidase subunit I. Earlier phylogenetic analyses of Lumbriculidae are
based on morphological characters or include a small number of taxa if based on
molecular data. Our results shed light on several questions: (i) the controversial
Dorydrilus michaelseni is placed within the Lumbriculidae, which implies that the
plesioporus male duct of Dorydrilus would be a simplification of the
semiprosoporous male duct of Lumbriculidae; (ii) the Trichodrilus with bifid
chaetae and male pores in IX, i.e. T. campoyi, T. diversisetosus, T. strandi and an
undetermined Trichodrilus, form a separate clade from the Trichodrilus with
simple pointed chaetae and male pores in X; (iii) Stylodrilus lemani is recovered
within Eclipidrilus and thus, morphological similarities between Stylodrilus and
Eclipidrilus are discussed; and (iv) the monophyly of several genera such as
Eremidrilus is recovered. Moreover, our study highlights which parts of the tree
require further taxonomic refinement and which ones are well-supported by morphological and molecular criteria.
15
ABSTRACTS
POSTER
^
Aquatic Oligochaeta (Annelida) Lakes of Aşagi Firat River Basin,
Turkey
Seval Aras (1) & Özlem Findik (2)
(1) Nevsehir Haci Bektaş Veli University, Faculty of Engineering and Architecture,
Department of Environmental Engineering, Nevsehir, Turkey; (2) Nevsehir Haci
Bektaş Veli University, Faculty of Arts and Sciences, Department of Molecular
Biology and Genetic, Nevsehir, Turkey
^
^
Aşagi Firat River is located in the south-east Anatolia region in Turkey. Aşagi
Firat River Basin is under the threat of pollution primarily originating from
several domestic point sources and agricultural sources. Samples were collected
from eight lakes at littoral area by hand-net 2014 summer and 2015 autumn.
According to the results, Oligochaeta rates of all the benthic invertebrates in
sampled lakes respectively were; Hacihidir Dam lake (62.9%); Atatürk Dam lake
(49.6%); Üçöz Dam lake (28.9%); Dumluca Dam lake (22.01%); Seve Dam lake
(13.9%); Çat Dam lake (8.5%); Karakaya Dam lake (7.8%) and Birecik Dam lake
(5%). A total of 1044 specimens of Oligochaeta were identified and eleven
species belonging to the Naididae and Tubificidae families. The most abundant
taxa were Limnodrilus hoffmeisteri (48.94%), Potamothrix hammoniensis (19.3%),
Tubifex tubifex (13.1%), Nais simplex (5.2%) and Nais communis (4.1%).
16
ABSTRACTS
ORAL
The use of BMWP and ASPT indices for evaluation of water quality
according to macroinvertebrates in Küçük Menderes Stream
(Turkey)
Naime Arslan (1), Ali Salur (2), Hasan Kalyoncu (3), Deniz Mercan (1) & Burcu
Barişik (1)
(1) Department of Biology, Faculty of Arts and Sciences, Eskisehir Osmangazi
University, Eskisehir, Turkey; (2) Department of Biology, Faculty of Arts and
Sciences, Hitit University, Çorum, Turkey; (3) Department of Biology, Faculty of
Arts and Sciences, Süleyman Demirel University, Isparta, Turkey
Monitoring studies that belong to benthic invertebrates in Küçük Menderes
Basin was occurred on May, July and September 2014 at totally 10 stations
(7 freshwaters and 3 standing water) according to method of Intercalibration
Common Metrics (ICMi) by suitable equipment for searching multihabitat every
station. All samples were identified at species level. Taxonomic composition,
individual number, and individual number at m2 evaluated theoretically, dominancy, frequency, existence of sensitive species, Shannon Diversity Index, Simpson
Index, Evenness values, Margalef Index, values of BMWP, ASPT and FBI at every
station were calculated. At the end of research, the highest taxonomic diversity
and highest individual number at 10 stations sampling biologically in Küçük
Menderes Basin were determined at a fluvial stations. It was calculated taxa
number between 1 and 22, individual number between 4 and 1149, Simpson
values between 0.6 and 0.9, Shannon Diversity Index between 3.391 and 1.706,
Evenness values 0.3 and 1, Margalef Index values between 0.32 and 2.41, FBI
values between 5.9 and 8.12, BMWP values between 5 and 38, ASPT values
between 2.5 and 5.37. Physicochemical parameters were showed parallelism with
FBI values. The highest taxa having frequency rate in basin-wide were
L. hoffmeisteri (70%), C. (Camptoch.) tentans (70%), P. albicola (60%), P. acuta
(60%), N. elinguis (60%) and S. lacustris (50%) which are alpha mesosaprobic and
polysaprobic species, respectively. Existence of these species and high dominancy
and frequency rate have indicated basin pollution. Positive indicator species are
G. schneideri, T. annulata, L. tetraphylla, O. cancellatum, H. angustipennis, C. (C.)
fuscus and C. (C.) annulator, while negative indicator species are C. pipiens, C.
(Camptoch.) tentans, C. thummi, S. lacustris and E. tenax.
17
ABSTRACTS
POSTER
Carpathian spring fens versus nearby brooks: what does
Trichodrilus strandi (Lumbriculidae) like more?
Martina Bílková & Jana Schenková
Department of Botany and Zoology, Masaryk University, Brno, Czech Republic
Trichodrilus strandi Hrabě, 1936, likewise other congeners, belongs to the
stygophilous species. There was little known about this species in the Czech
Republic and Slovakia, but recent field research of treeless spring fens (with
various geographical position, size, altitude, vegetation, mineral richness of
water, substrate etc.) in the Western Carpathians showed that Trichodrilus strandi
prefers mineral-rich fens with tufa formation and its populations reach high
abundances replacing other oligochaete species there. Later research of nearby
brooks revealed that this species inhabited also adjacent brooks but only in waters
with alkaline reaction. The aim of our study was to compare populations of
Trichodrilus strandi living in spring fens and in nearby brooks. Material was
collected in spring and autumn seasons (2006–2013), in spring fens at two mesohabitats (standing water and flowing water), in brooks at three mesohabitats
(riffle, pool, mosses). Main factors determining the presence of Trichodrilus
strandi were identified. Some differences between these two main types of
habitat and also between individual mesohabitats were observed. Supported by
GA15-15548S, MUNI/A/0888/2013.
18
ABSTRACTS
ORAL
Macroecological patterns of macrozoobenthos in rivers of China
Yongde Cui, Weihua Zhao & Hongzhu Wang
Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China
Macroecological patterns and environmental influence of macrozoobenthos
were investigated using field-data collected from seven rivers and data available
in the literature of China. Macroecological patterns of macrozoobenthos
distribution and the influencing factors in rivers of China were systematically
analyzed for the first times. A total of 171 genera of macrozoobenthos were
recorded from 46 rivers, representing oligochaetes (11.5%), molluscs (17%),
insects (69%) and other animals (3.5%). Macrozoobenthos assemblages varied
regionally (Northern, Southern, Western and Eastern) on the basis of species
composition of macrozoobenthos. Taxa number decreased in most of the rivers in
recent years, except for some seriously polluted rivers. Macrozoobenthos density
was much higher in small rivers than larger and mid-larger rivers, while biomass
in large and small rivers was much lower than in mid-larger rivers. The densities
in most rivers were higher in upper and lower reaches than in middle reaches,
while biomass in middle and lower reaches were higher than in upper reaches.
Macrozoobenthos also decreased in recent year on the basis of density and
biomass. The densities and biomass were highest in autumn and winter. Aquatic
insects usually dominated assemblages in the mid and lower reaches of large
rivers, while oligochaetes and molluscs dominated the standing crop. Molluscs,
echinoderms, polychaetes and crustacean dominated assemblages at the river
mouths. The predominant groups have been changed or changing in some rivers
due to pollution. At large spatial scales, altitude, precipitation, drainage area,
river length and macrophytes all were influential in determining assemblage
characteristics, while the most important artificial influence factors were some
altered water quality parameters by pollution and altered substrate by hydraulic
engineering.
19
ABSTRACTS
ORAL
Alien species from selected annelids families and their role in
aquatic habitats in Poland
Elzbieta Dumnicka
Institute of Nature Conservation, Polish Academy of Sciences, Krakow, Poland
Non-indigenous species from families Naididae and Aeolosomatidae have
been reported from Poland. Naididae species of various origin (Quistadrilus
multisetosus, Limnodrilus cervix, Branchiura sowerbyi, Tubifex blanchardi,
Potamothrix bavaricus, P. vejdovskyi, P. heuscheri, Psammoryctides moravicus and
Paranais simplex) have been found in a small number of locations and usually in
a limited number of specimens. Some species of the Ponto-Caspian origin
(Potamothrix hammoniensis, P. moldaviensis, Psammoryctides barbatus and
P. albicola) are common and frequently numerous in various habitats. Up to now
only four last mentioned species could be treated as invasive ones. It cannot be
excluded, that these species had lived in water bodies of Central Europe before
the last glaciation epoch and recolonized this habitat naturally. Maybe future
studies of subfossil sediments could determine more precisely the time of their first
occurrence in this region. The influence of these common and numerous species
on abiotic and biotic elements of ecosystems could be significant, especially in
eutrophic lakes and slowly running rivers. Little is known about the origin of
species belonging to the genus Aeolosoma and their distribution in Poland.
Termophilous species (A. flavum, A. sawayi) and A. japonicum, known only from
heated lakes, are probably recent immigrants but other species from this genus
could be native or introduced to European water bodies even in the XIX. century.
The number of alien oligochaete species recorded in Poland has increased
considerably in the last 50 years. This might be due to more intensive studies of
oligochaete fauna as well as due to new introductions. Non indigenous species
have been noted mainly in man-made or altered by man water bodies (dam
reservoirs, artificial ponds, heated lakes) although some species have been also
found in natural water bodies, especially in western part of Poland (the drainage
area of Oder River) named “gate for alien species”.
20
ABSTRACTS
ORAL
Systematics of Lumbricillus (Clitellata, Enchytraeidae) using
molecular and morphological approaches
Ma° rten Eriksson, Christer Erséus & Svante Martinsson
Department of Biological and Environmental Sciences, University of Gothenburg,
Göteborg, Sweden
The enchytraeid genus Lumbricillus comprises about 80 described species,
which are up to a few centimeters in length, and inhabits mostly the littoral zone
of non-tropical marine environments world-wide. The phylogeny of this genus is
poorly studied, but previous work has shown Lumbricillus to be a nonmonophyletic group. In this study, the phylogeny of this genus is re-estimated
using more than 300 specimens from 24 species (out of which 20 have been
identified with nominal names), samples of which have been sequenced for three
mitochondrial and four nuclear molecular markers. DNA-barcoding was used
together with statistical and coalescent based applications for species
delimitation. Gene trees, concatenations and multispecies coalescent based
species trees were estimated using Bayesian inference. I found most of the 24
species as well-supported, and two possible cases of cryptic speciation.
Furthermore, the estimated phylogenies confirm a non-monophyletic Lumbricillus
containing a monophyletic Lumbricillus sensu stricto in which L. semifuscus is
clearly excluded. I also found inconclusive evidence suggesting that L. arenarius,
L. dubius as well as an unidentified species should be transferred into a new
genus, in order to ensure a monophyletic Lumbricillus sensu stricto. Finally,
I provide illustrated morphological re-descriptions of all 24 included species.
21
ABSTRACTS
KEYNOTE LECTURE
From DNA barcoding and species delimitation to transcriptome
phylogenies - on ongoing molecular systematics research on
clitellates
Christer Erséus
Göteborg, Sweden
This presentation will give a status report of molecular approaches at various
levels of the systematics of Clitellata. DNA barcoding has been claimed to provide
a useful tool for species level taxonomy and identification, but if uncritically used
without prior knowledge of intra-specific variation in each taxon case, it is a
method with pitfalls. Nevertheless, it will be argued that the commonly used
barcoding gene COI (mitochondrial DNA) is a valuable descriptor for clustering
specimens according to their maternal history, which may serve as an important
step towards species delimitation. Species boundaries, however, should be
established by an integrative approach, including also nuclear DNA data, and
when possible, morphological or other information. A species hypothesis can be
genetically supported either by congruence between gene trees or by coalescent
analysis. Cryptic species will also be discussed; they will here be defined as cases
where morphological data alone do not provide reasonably convincing evidence
of speciation. Finally, recent achievements in the estimation of clitellate
phylogenies will be exemplified, including preliminary results of analyses of
transcriptomic data generated by “Next Generation Sequencing”.
22
ABSTRACTS
ORAL
Annelids in hypereutrophic Upper Klamath Lake, Oregon, USA
Steven V. Fend (1), James L. Carter (1) & Natalie Stauffer-Olsen (2)
(1) US Geological Survey, 345 Middlefield Rd. Mailstop 496, Menlo Park,
California 94025, USA; (2) Environmental Science, Policy, and Management,
Wellman Hall, University of California, Berkeley, California 94702, USA.
Upper Klamath Lake is a large (232 km2), shallow (mean depth 2.4 m)
freshwater lake in southern Oregon, USA. The lake has been eutrophic for much
of its history, but is currently considered hypereutrophic, with summer
cyanobacteria blooms resulting in localized hypoxia. Aquatic annelids were the
dominant benthic invertebrates collected during several studies (2007-present)
aimed primarily at estimating contributions of invertebrates to nutrient flux
between sediment and water column. These studies have documented lake-wide
spatial and temporal distributions, and colonization of restored wetlands. As in
some other western Nearctic lakes, Ilyodrilus frantzi was a dominant species. The
widespread Dero digitata and Aulodrilus pigueti, and the polychaete Manayunkia
sp. were locally abundant. Other oligochaetes in Upper Klamath Lake differed
from more widespread congeners in the surrounding watershed: a “giant”
Varichaetadrilus sp. vs. V. pacificus, Limnodrilus cf. spiralis vs. typical
L. hoffmeisteri, Rhynchelmis klamathensis vs. R. rostrata, and a “dwarf” Altmanella
sp. vs. typical A. freidris. Three unusual leeches (Helobdella bowermani and two
possibly-new Helobdella species) dominated open-water lake habitats, in contrast
to H. modesta/stagnalis in the watershed. Lumbriculids were seasonal, with
R. klamathensis reproducing in the autumn and Altmanella sp. in the spring.
Although the tubificines may have reproduced throughout the year, mature
Limnodrilus sp. were most abundant in late summer, and I. frantzi in spring.
Sexually-mature Varichaetadrilus sp. were rarely collected. The benthos of most
open-water sites appeared more homogeneous than that of littoral sites, and
dominant oligochaetes were I. frantzi and Varichaetadrilus sp. High densities of
Limnodrilus sp. occurred in a deep (15 m) trench, but the lumbriculid species were
absent there, possibly due to local hypoxia.
23
ABSTRACTS
POSTER
Aquatic Oligochaeta (Annelida) of Ceyhan River Basin's Lakes
(Turkey)
Ozle Findik (1) & Seval Aras (2)
(1) Nevsehir Haci Bektas Veli Unv. Arts and Sciences Faculty, Moleculer Biology
and Genetics Department, Nevsehir Turkye; (2) Nevsehir Haci Bektas Veli Unv.
Enginering faculty; Nevsehir Turkye
Oligochaeta samples were collected from seven lakes (2 natural and 5 nonnatural) of Ceyhan River Basin at 2014 summer and 2015 autumn. Ceyhan river
basin is located in the South Anatolia region in Turkey. Gölbaşi and Azapli lakes
are protected area. Some physico-chemical parameters of surface water were
measured in situ. Sampling were done by hand-net and ekman grap at different
depth. Oligochaeta was the dominant group of the total benthic invertebrates
expect Ayvali Dam lake. A total of 11293 Oligochaeta samples were examined
and 13 species of Oligochaeta belonging to 2 families (Naididae and Tubificidae)
were recorded from lakes. Potamothrix hammoniensis (90.24%) and Limnodrilus
hoffmeisteri (6.65%) were the highest species of total Oligochaeta.
24
ABSTRACTS
ORAL
Life in a toxic environment - an exceptional new "sulphur worm" of
the genus Limnodrilus
Olav Giere (1), David Steinmann (2), Stephan Scholz (3), Christian S. Wirkner
(3), Nadja Hellmann (4) & Ulrich Höger (5)
(1) Zoological Institute and Zoological Museum, Univ. of Hamburg, Hamburg,
Germany; (2) Denver Museum of Nature & Science, Denver (Co.), U.S.A.; (3)
Institute for Biological Sciences, University of Rostock, Rostock, Germany; (4)
Institute of Biophysics, University of Mainz, Mainz, Germany; (5) Institute of
Zoology, University of Mainz, Mainz, Germany
Rich populations of a new Limnodrilus (Naididae; to be described elsewhere)
thrive in a shallow thermo-mineral creek in Sulphur Cave (Colorado, U.S.A.)
where they are exposed to high concentrations (> 1 mg.l-1 or >30 mikroM) of toxic
hydrogen sulfide. Our studies revealed marked structural and physiological
adaptations to these hostile ecophysiological conditions in comparison with
L. hoffmeisteri from typical lake habitats:
- a dense network of anastomosing vessels in the epidermal layer,
- a massive chloragogue tissue covering wide central blood vessels and the
intestinal blood sinus,
- rich aggregations of 'brown pigment' consisting of voluminous chloragocytes
filled with chloragosomes,
- hemin and hemin-metabolites in brown pigment,
- high sulphur concentrations in chloragosomes,
- elevated thiosulphate concentrations in worm tissue upon exposure to sulfide,
- blood with high binding capacity for oxygen,
- no evidence for the presence of symbiotic sulphide oxidizing bacteria
From these data we derive the following ecophysiological model:
In order to utilize the dense mats of sulphur bacteria in the cave, the Limnodrilus
population has to be able to bind enough oxygen from the hypoxic water and,
simultaneously, detoxify the noxious sulfide diffusing into the body. This is
ascertained by an allosteric binding of both components at the haemoglobin
molecule. A non-competitive sulphide binding with separate free cysteine sites
has been shown earlier to occur in various annelid species from sulfide-rich
environments. A dense peripheral network of tiny blood vessels connects the body
surface with the central blood system and its surrounding chloragocytic tissue.
While the bound oxygen is further channelled to the respiration chain, the sulfide
load of the haemoglobin molecules is delivered to the chloragosomes. Here, the
heme containing 'brown pigment' serves as a detoxifying substance catalysing the
oxidation of sulfide into non-toxic thiosulfate as shown before for other
invertebrates. This oxidation product easily permeates the body tissues for
excretion into the water.
Although novel for freshwater animals, this scenario could explain the findings
documented above and the existence of these Limnodrilus populations in a
hostile environment.
25
ABSTRACTS
POSTER
Ovary structure in basal Oligochaeta
Szymon Gorgoń (1), Bartosz J. Płachno (2), Richard Marchant (3), Mariola
Krodkiewska (4), Karol Małota (1), Anna Z. Urbisz (1) & Piotr Świątek (1)
(1) Department of Animal Histology and Embryology, University of Silesia,
Katowice, Poland; (2) Department of Plant Cytology and Embryology, Jagiellonian
University in Kraków, Kraków, Poland; (3) Museum Victoria, Melbourne, Australia;
(4) Department of Hydrobiology, University of Silesia, Katowice, Poland
Classical and recent studies that have been devoted to the ovary structure and
oogenesis in Clitellata showed that the morphology of the ovaries varies between
taxa and their organization is specific at the family level. Despite the different
morphological organization, there are some common features. One of them is the
formation syncytial cysts of interconnected germ cells in early oogenesis. The
pattern of cyst organization is the same in all clitellates; each cell is connected to
the central common anuclear cytoplasmic mass, the cytophore, by one stable
intercellular bridge. During cyst development, the germ cells differentiate into two
morphologically different subpopulations – less numerous oocytes and more
numerous nurse cells, i.e. cells that contribute to the oocyte development. One of
the groups among Clitellata in which the ovary structure was completely unknown
is the family Capilloventridae. This taxon is regarded as a sister group to all of the
other clitellates. We described the gonad organization in Capilloventer australis at
the ultrastructural level. Generally, the ovaries in C. australis are paired structures;
each one is built of a linearly arranged row of growing germ cells. Oogonia and
previtellogenic oocytes can be found in the apical part of the ovary. The next part
of the ovary is occupied by oocytes that just started to gather reserve material
(early vitellogenic oocytes). Vitellogenic oocytes (tightly packed with yolk) detach
from the ovary and fill the segment lumen. To our surprise we found that no
germ-line cysts are formed in the ovaries of C. australis. Female germ cells are
individual during all of the stages of oogenesis and all of the germ cells have the
potential to develop into oocytes. Such an ovary organization and the mode of
oogenesis are unusual for Clitellata. Research was funded by the grant from the
National Science Centre, Poland, DEC-2012/05/B/NZ4/02417.
26
ABSTRACTS
ORAL
Profundal benthic macroinvertebrates in Japanese lakes of
different trophic status
Kimio Hirabayashi (1) & Akifumi Ohtaka (2)
(1) Applied Biology, Academic Assembly, Shinshu University, Ueda, Nagano,
Japan; (2) Faculty of Education, Hirosaki University, Hirosaki, Japan
Profundal benthic macroinvertebrates were investigated in fifty freshwater
Japanese lakes of different trophic status. The sampling surveys were carried out
using a standard Ekman grab at the lake center. We measured densities
(individual number/m2) and biomass (wet weight/m2) of the benthic
macroinvertebrates in the profundal zone. Chironomid larvae and oligochaete
were dominant organisms in the profundal zone of the lake. We analyzed the
relationship among the chironomid larvae, oligochaetes density and biomass in
these lakes. As a result, there was no relationship between the biomass of
chironomid larvae and oligochaetes. However, the lakes were classified into four
categories by the pattern of occurrence of chironomid larvae and oligochaetes,
i.e., (1) both chironomid larvae and oligochaetes were abundant (OligochaeteChironomidae Type). (2) Chironomid larvae were also abundant, but there were
only a few oligochaetes (Chironomidae Type). (3) Oligochaetes were abundant,
but there were only a few chironomid larvae (Oligochaete Type), and (4) only a
few of both chironomid larvae and oligochaetes present. Lake Akan and Lake
Teganuma typically had the Chironomidae Type, and Lake Kizaki and Lake Yunoko
had the Oligochaete Type. But almost all of the lakes were the OligochaeteChironomidae Type. According to Ohtaka (2014), there was a relationship
between the occurrence of oligochaete species and lake water trophic status. This
study is focusing on the relationship between the occurrences of oligochaete
species (e.g., Tubifex tubifex, Limnodrilus hoffmeisteri, etc.) and chironomid
species (e.g., Propsilocerus akamusi, Chironomus plumosus, Chironomus
nipponensis, etc.) in Japanese lakes of different trophic status.
27
ABSTRACTS
ORAL
Research on the seasonal variation of biomass of marine
oligochaeta Thalassodrilides sp. with high pollution tolerance in
Fukuura Bay, Japan and its efficient culture condition
Katsutoshi Ito (1), Mana Ito (1), Kohei Ohta (2), Toshimitsu Onduka (1), Takeshi
Hano (1), Nobuyuki Ohkubo (1) & Kazuhiko Mochida (1)
(1) National Research Institute of Fisheries and Environment of Inland Sea,
Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452,
Japan; (2) South Ehime Fisheries Research Center, Ehime University, 1289-1,
Funakoshi, Ainan, Ehime 798-4292, Japan
It is well known that aquatic annelids have high resistance to environmental
pollution compared with other benthos. We have been studying environmental
remediation using annelids and demonstrated that aquatic annelids have a high
potential to lower the concentration of pollutant chemicals. For the establishment
of environmental remediation using annelids in natural environment, it is
necessary to grasp life cycle of suitable species in the environment. In this study,
we investigated the seasonal variation of dominancy of a marine oligochaeta
(Tubificidae) Thalassodrilides sp., which has high tolerance to pollutant, in
benthic community in Fukuura Bay. In addition, growth rates of the oligochaetes
under the different temperatures was examined to establish an efficient culture
system in the laboratory. In the field studies, we investigated the abundance and
biomass of the oligochaete four times a year according to four seasons from 2011
to 2014 in Fukuura Bay, Ehime Prefecture, Japan. Throughout the investigation
period, abundances of Thalassodrilides sp. were dominated more than 60% of the
total abundance of benthic communities except in winter of 2013 and 2014, and
it reached 121,400 individuals / m2 in February 2012. From spring to summer, the
average body weight (/individual) reached was maximum and the proportion of
mature individuals was the highest in summer. These results suggest that
alternate generation of this species occurs in summer. In the laboratory
experiment, Thalassodrilides sp. was cultured at 15, 20, or 25°C, and was
measured the growth rate and maturity rate. The growth rate and the proportion
of having egg sac after 30 days cultured at 15, 20, and 25°C were 130, 138, and
160%, and 1.4, 23, and 71%, respectively. It is considered that the temperature
rise to 20-25°C triggers maturation of this species and the culture at 25°C is the
most effective for inducing maturation.
28
ABSTRACTS
ORAL
Marine oligochaete, Thalassodrilides sp.; a potential candidate for
bioremediation of polycyclic aromatic hydrocarbons-contaminated
sediment
Mana Ito (1), Katsutoshi Ito (1), Kazuki Ito (1), Kohei Ohta (2), Toshimitsu
Onduka (1), Takeshi Hano (1), Nobuyuki Ohkubo (1) & Kazuhiko Mochida (1)
(1) National Research Institute of Fisheries and Environment of Inland Sea,
Fisheries Research Agency, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452,
Japan; (2) South Ehime Fisheries Research Center, Ehime University, 1289-1,
Funakoshi, Ainan, Ehime 798-4292, Japan
In some coastal areas, sediment have been contaminated by various chemical
compounds, which cause some significant threats on marine organisms.
Therefore, it is important to develop a remediation technique. We have focused
bioremediation with marine benthos such as aquatic oligochaete. In this study, we
investigated if the marine oligochaete, Tharassodridides sp. could reduce the
concentration of polycyclic aromatic hydrocarbons (PAHs) in the
PAHs-contaminated sediment, and analyzed their biological response of the
oligochaete at exposure to the sediment. After 50 d exposure of Thalassodrilides
sp. to the PAHs-contaminated sediment, the concentrations of 16 PAHs contained
in the sediment were analyzed by GC/MS. The total concentration of 16 PAHs in
the sediment with the oligochaete was significantly lower than those in the
sediment without the oligochaete. This result suggests that the oligochaete has
high potential for bioremediation in oil polluted sediment. A transcriptome
analysis was conducted to reveal the biological response after the exposure to the
PAHs-contaminated sediment. After 10 d exposure to the sediment, the gene
expression of cytochrome P450 monooxygenases which involved in the
metabolism of the xenobiotics was up-regulated approximately 10-fold compared
with the initial level. The gene expression levels were also examined after
10 d exposure to the sediment at different temperature, such as 15, 20, or 25°C.
The gene expression at 20°C was 5.5- and 3.8-fold to those at 15 and 25°C,
respectively, suggesting that the optimum temperature for degradation of PAHs is
around 20°C.
29
ABSTRACTS
ORAL
Aquatic oligochaetes community in AL-Dalmage lake/ Iraq
Haifa J. Jaweir & Maysoon Hasaan Al-Sarai
Department of Biology, College of Science for Women, University of Baghdad,
Iraq
Al-Dalmage Lake is a part of middle section of main outfall drain, and which
is located on southern Baghdad / Iraq. This study concerned with the community
structure of aquatic oligochaetes in this lake, in addition to the physic-chemical
properties of the lake water. Six stations along the eastern side of the lake were
chosen for samples collection, for a period from Jan. 2013 to Jan. 2014. The
results revealed that the lake water is oligohaline with salinity reached 14.6‰
and the temperature range between 9-33°C. pH values range between 7-9.
Water was well aerated with dissolved oxygen concentrations values ranged
between 7-12 mg/l, and BOD values between 1.5-3mg/l. The lake water was
considered as very hard, since the total hardness values ranged between 8256880 mg/l. Total of 1885 individuals of aquatic oligochaetes were sorted during
study period from all sites, 58% of them belong to family Aeolosamatidae, 36%
to Tubificid worms and 6% to Naidid worms. Two aeolosomatid species were
recognized, Aeolosoma hemprichi and Aeolosoma variegatum. Naidid worms of
sub family Naidinae including three species, Paranais litoralis, Dero (Aulophorus)
furcata, Stylaria lactustris. The highest frequency percentage of 23.6% was
recorded by P. litoralis. According to the Tubificid worms, it was represented by six
species, Limnodrlius hoffmeisteri, L. profundicola, L. claparedeianus,
L. udekemianus, Tubifex tubifex and Branchiura sowerbyi. L. claparedeianus was
the most abundant species which comprised 53% of total number of
Oligochaetes. Seasonal flactuations of different species of oligochaetes worms
were also determined. It was also noted that there were also a spatial and
temporal variations of species richness. The highest species richness was
recorded in S1 and during June and October 2013.
30
ABSTRACTS
ORAL
Advantages of DNA-barcoding for understanding of the species
diversity within freshwater annelids of Eastern Siberia
Irina A. Kaygorodova
Limnological Institute, Irkutsk, Russia
Leeches compose a relatively small group of highly specialized annelids.
Currently no more than 700 species are known worldwide. In fresh and brackish
waters, some leeches serve as invertebrate predators while others are infamous
for their ability to feed on blood either invertebrates or even vertebrates. Both
parasitic and non-parasitic leeches are one of the most sophisticated biological
objects for taxonomical determination. In connection with insufficient knowledge
on the Hirudinea fauna coupled with existence of cryptic and morphologically
inexpressive species, their morphological identification has been often
complicated. That is why the species identification of leeches was and still is
highly doubtful and needs to be clarified. Since the emergence and successful
implementation of DNA barcoding techniques into biology, it became possible to
wider use molecular data in faunal and environmental studies. Sequencing of
particular mitochondrial genes in animals, such as the COI gene, can yield
phylogenetic information as well as aid in the identification of species. Application
of molecular ecology methods, which are not sufficient to determine the rank
taxa, however, allow to evaluate the degree of genetic relatedness of species in
comparison with the existing classification. In this study, COI sequences of leech
species were obtained for the first time from specimens collected in different
freshwater reservoirs within Eastern Siberia including Lake Baikal. Using these
sequences, phylogenetic interrelationships were traced out. Genetic diversity was
revealed and genetic distances were evaluated. DNA barcodes were generated
for newly sequenced species of leeches from Eastern Siberia. Morphological data
were used to corroborate DNA identification. As a result, leech fauna of Eastern
Siberia has been refined and augmented. Currently, the species list was at least
doubled.
31
ABSTRACTS
ORAL
Oligochaete communities in anomalous regions of Lake Baikal,
Eastern Siberia, Russia
Irina A. Kaygorodova
Limnological Institute, Irkutsk, Russia
The oligochaete fauna of two anomalous parts of Lake Baikal were studied,
including underwater hydrothermal vent affected zone of the Frolikha Bay
(Northern Baikal) and natural oil seep at Cape Gorevoi Utes (Middle Baikal).
Biological material was collected during the dives of manned deep-diving
submersible "Mir-1" and "Mir-2" in August 2009. This study provides information
about a unique community of oligochaetes inhabiting there. An updated checklist
of abyssal oligochaete species of Frolikha Bay consists of 23 species including 21
species inhabiting areas adjacent to the vent. These species belong to 11 genera,
which relate to three Oligochaeta families, of them Naididae sensu Erséus et al.
(2008) is represented of 19 species, Propappidae – 1 species and 1 species of
Lumbriculidae. Among Naididae species belonging to three subfamilies were
found: Naidinae (one form of uncertain species status), Tubificinae (12 species)
and Rhyacodrilinae (6 species). Uncharacteristic for the abyssal zone of Lake
Baikal high indices of oligochaete development were revealed in area of Frolikha
underwater hot seeping. Unique features of Lake Baikal include natural oil seeps
in its shelf zone. Near Cape Gorevoi Utes oil occurs as bitumen in shore cliffs or
rises from the lake bottom as floating spherules of viscous hydrocarbons. In the
area of oil shows, we found 24 species of oligochaetes, of them 15 species live
directly on the substrate with bitumen inclusions. These species belong to 10
genera and 2 families of Oligochaeta. Of them, former Tubificidae, including
Tubificinae and Rhyacodrilinae - 9 species, and Lumbriculidae – 6 species. Neither
Propappidae, no Enchytraeidae were detected. Quantitative indicators of
oligochaetes are not high, which is typical of the abyssal zone of the lake.
However, four species previously recorded only in the littoral zone of Lake Baikal
are recorded in the oil communities.
32
ABSTRACTS
POSTER
The use of biological indices based on bottom oligochaetes in the
assessment of restoration measures in an anthropogenic reservoir
(Pławniowice Reservoir, southern Poland)
Mariola Krodkiewska (1) & Maciej Kostecki (2)
(1) Department of Hydrobiology, Faculty of Biology and Environmental Protection,
University of Silesia, Katowice, Poland; (2) Institute of Environmental Engineering
PAN, Zabrze, Poland
The Pławniowice Reservoir is an anthropogenic water body that was created in
1974 through the flooding of a sandpit by the Toszecki Stream. In the 1980s and
the 1990s, the reservoir was degraded due to eutrophication, and therefore,
restoration using selective hypolimnetic withdrawal has been applied since 2003.
The studies were carried out in 2004, 2008 and 2011 (the first, fifth and eighth
year of the restoration). Samples were collected during the summer stratification
at twenty-two sites along six transects that covered depths from two to fifteen
meters. The aim of the survey was to estimate whether indices based on
oligochaetes may be useful in the assessment of a reservoir’s recovery from
eutrophication. The results that were obtained indicated that there was an
improvement in some water parameters (i.e. an increase in the water
transparency and dissolved oxygen concentration near the bottom in the deepest
area of the reservoir, a reduction in the orthophosphate concentration and a
decrease in the pH of the surface layer in summer) in respect to those parameters
during the period prior to the restoration measures. The study also showed an
increase in the oligochaete diversity and a decrease in their abundance, which
was accompanied by a shift towards a larger share of taxa that are characteristic
of mesotrophic and oligotrophic conditions. During the period of the study, the
values of the trophic indices based on oligochaetes indicated changes from a
eutrophic to an almost mesotrophic state of the reservoir. Discriminant analysis,
which was performed using stepwise forward selection, demonstrated a good
separation of the samples from 2004 and 2011 according to the TC and BQI
indices. The study shows that the use of bottom oligochaetes in biological metrics
may constitute a valuable tool in detecting changes in the trophic status of
aquatic habitats. This is especially important due to the continual eutrophication
of freshwater environments.
33
ABSTRACTS
POSTER
Diversity of Oligochaeta in Korea
Jeounghee Lee (1) & Jongwoo Jung (1,2)
(1) The Division of EcoCreative, Ewha Womans University, Seoul 120-750, Korea;
(2) Department of Science Education, Ewha Womans University, Seoul 120-750,
Korea.
Oligochaeta inhabits many diverse environments that seawater, freshwater
and groundwater, as well as clean water to polluted water. Korea is surrounded
by the ocean on three sides, and has many lakes, streams, rivers, and valleys.
Aquatic oligochaetes in Korea have received but scant attention. Recently,
several researchers have already found 7 families, 30 genera and 42 species.
We newly found six species from April 2013 to September 2014.
34
ABSTRACTS
ORAL
Extensive cryptic diversity and taxonomic confusion in the
cosmopolitan Limnodrilus hoffmeisteri (Clitellata, Naididae)
Yingkui Liu & Christer Erséus
Department of Biological and Environmental Sciences. University of Gothenburg,
Gothenburg, Sweden
In contemporary taxonomic studies, the worm Limnodrilus hoffmeisteri
Claparede, 1862 (Annelida, Clitellata, Naididae) is generally seen as a single, but
variable, cosmopolitan species. Its great phenotypic plasticity has resulted in a
long history of complex taxonomy, including many names today regarded as
junior synonyms of Limnodrilus hoffmeisteri. A new, integrative approach that
compared molecular analyses with traditional morphological identification tried to
resolve this issue. The species diversity of L. hoffmeisteri was firstly investigated by
analyzing a partial mtDNA COI (cytochrome oxidase subunit 1) data set.
Sequence variation in the COI data set was strongly partitioned up to 29 putative
species, the majority of these were also supported by analysis another
mitochondrial 16S ribosomal RNA data set and a nuclear ITS marker data set.
However, the molecular data were incongruent with groups indicated by the
morphological evidence. The results presented here contribute to the clarification
of the previously noted confusion in Limnodrilus taxonomy; L. hoffmeisteri is
likely to be a complex of more or less cryptic species. The study also evaluates the
former, morphology-based taxonomic characteristers in a phylogenetic species
context and even identifies new features suitable for the differentiation of
Limnodrilus species.
35
ABSTRACTS
ORAL
The groundwater oligochaetes (Annelida, Clitellata) from the "Parc
du Mercantour" (France)
Patrick Martin (1), Michel Creuzé des Châtelliers (2), Rüdiger Schmelz (3) &
Marie-José Olivier (4)
'
'
(1) Institut royal des Sciences naturelles de Belgique, Biologie des Eaux douces,
29 rue Vautier, B-1000 Brussels, Belgium; (2) Université de Lyon, UMR5023
Ecologie des Hydrosystemes naturels et anthropisés, Université Lyon 1, ENTPE,
CNRS, 6 rue Raphaël Dubois, 69622 Villeurbanne, France; (3) ECT
~
Oekotoxikologie GmbH, 61459 Flörsheim, Germany & Universidad de A Corun
a,
Fac. Ciencias, Dep. Biología Animal, Biol. Vegetal, y Ecología, Rua da Fraga 10,
~
E-15008 A Corun
a, Spain; (4) Université Lyon 1, CNRS, UMR 5023 - LEHNA,
Laboratoire d'Ecologie des Hydrosystemes Naturels et Anthropisés, Bât. Forel,
F-69622 Villeurbanne Cedex, France
Although recognized as an outstanding hotspot of biodiversity for both flora
and fauna, the Mercantour massif remains almost totally unexplored for its
groundwater fauna. This work reports the first overview on groundwater
oligochaete assemblages of the "Parc du Mercantour" after a standardized
exploration of both consolidated (fractured massif) and unconsolidated (porous)
aquifers. About 40 species of oligochaetes were found in 49 stations
representative of the main hydrogeological basins of the "Parc du Mercantour",
from both spring and hyporheic zone habitats. Five stygobiont species are
identified, probably all new to science, of which two species are formally
described, Aberrantidrilus stephaniae n. gen., n. sp. (Naididae, Phallodrilinae) and
Marionina sambugarae n. sp., a species belonging to the widespread Marionina
argentea species complex (Enchytraeidae). As a result, the genus Abyssidrilus
Erséus, 1992 is now restricted to its marine, abyssal species. A dozen of species
can be considered as stygophiles. Most stygobiont species are recorded from
hyporheic habitats, and stygophiles have a more balanced distribution between
both kinds of habitats. The nearly absence of stygoxene species from the
hyporheic zone suggests that this habitat is less affected by the epigean
environment than springs. The dominance of enchytraeids among the
groundwater oligochaete fauna is here confirmed, and the lumbriculid genus
Trichodrilus is also a characteristic faunistic element of the underground
freshwater oligochaete communities. Lastly, the possibility that Aberrantidrilus
cuspis n. comb. sensu Sambugar et al. (1999) is a complex of cryptic species is
discussed in the framework of recent progress in the knowledge of groundwater
biodiversity, and following an integrative taxonomy approach (morphology and
molecular data).
36
ABSTRACTS
POSTER
Groundwater oligochaetes (Annelida, Clitellata) of the Dinaric
region
Enrique Martínez-Ansemil (1), Federica Giacomazzi (2) & Beatrice Sambugar †
(1) Departamento de Bioloxía Animal, Bioloxía Vexetal e Ecoloxía, Facultae de
~
~
Ciencias, Universidade da Corun
a, A Corun
a, Spain; (2) Museo Civico di Storia
Naturale, Verona, Italy
The Dinaric region is a karstic area extended to more than 56,000 km2
in southern Europe belt between the Adriatic sea and the NE hills and plains.
The isolated position of this region along with its tropical past and dynamic
geomorphologic changes allowed development of a rich endemic flora and
fauna, including the richest in the world hypogean fauna (Sket, 1997).
Several years ago, Professor B. Sket (University of Ljubljana, Slovenia) made
available to the late Beatrice a large collection of approximately 400 samples of
oligochaetes from caves, wells, springs, and interstitial areas of this region,
collected over many years (1960s through 1990s). Available historical data and
the identification of a part of this material (41 samples), along with 46 samples
from a campaign in Slovenian caves conducted by Dr. F. Stoch in the 1990's and
174 samples taken in different groundwater habitats of the Krim Massif area
during the European project PASCALIS at the beginning of the 2000's, allowed us,
with other colleagues, to make an overall study on the groundwater oligochaetes
of Slovenia (Giani et al., 2011).
The aim of this work is to enlarge knowledge of groundwater oligochaetes
in the Dinaric region, by identifying the 72 samples of Sket's collection taken
outside of Slovenia (Croatia, Bosnia-Herzegovina, Serbia, Montenegro and
Macedonia). More than fifty species have been identified, raising to 125 the total
number of species known at present in groundwaters of the overall Dinaric
region. More than 40% of these species are characteristic from groundwater
environments (i.e. 34 stygobionts and 19 stygophiles); most of them are
endemic. The 34 stygobiotic species now known to occur in the Dinaric region
represent nearly 1/3 of all stygobiotic freshwater oligochaete species known in
the world. The genera Trichodrilus, Rhyacodrilus, Epirodrilus, Rhyacodriloides,
Abyssidrilus, Spiridion, and Parvidrilus include the bulk of the most noteworthy
species.
37
ABSTRACTS
On the systematics
(Enchytraeidae)
ORAL
Chamaedrilus
and
Euenchytraeus
Svante Martinsson
Gothenburg, Sweden
The enchytraeid genus Cognettia was recently proposed to be split into its two
senior synonyms, Euenchytraeus and Chamaedrilus, the majority of species was
placed in Chamaedrilus whereas a few species with head nephridia was placed in
Euenchytraeus. Using molecular methods we have shown that the North European
fauna of Chamaedrilus consist of 8 species, instead of 4 as previously believed.
The species in the Chamaedrilus sphagnetorum and glandulosus complexes in
Northern Europe have been revised based on molecular and morphological data,
and we have described 3 new species. A neotype for the type species of Cognettia,
Pachydrilus sphagnetorum, and lectotypes for the type species of Chamaedrilus,
Ch. chlorophilus, and for Ch. glandulosus have been designated. Chamaedrilus
has been previously been found as the sister-group to Stercutus. However, so far
none of the species currently placed in Euenchytraeus has been included in any
phylogenetic studies, and its position with in Enchytraeidae is unknown. Further
the reciprocal monophyly of both Euenchytraeus and Chamaedrilus needs to be
tested. Here we address these questions with a molecular phylogeny of the
family Enchytraeidae, with focusing on the position of these two genera.
38
ABSTRACTS
ORAL
Metal tissue baseline concentrations in aquatic oligochaetes from
mining areas of Northern Spain
Leire Méndez-Fernández (1), Maite Martínez-Madrid (2) & Pilar Rodriguez (1)
(1) Dpt. Zoology and Animal Cellular Biology. University of the Basque Country.
Box. 644, 48080 Bilbao, Spain; (2) Dpt. Genetics, Physical Anthropology and
Animal Physiology. University of the Basque Country. Box. 644, 48080 Bilbao,
Spain
Metal bioaccumulation in benthic macroinvertebrates are part of an
integrative approach to ecological risk assessment in the Nalón River basin, where
sediment toxicity in several sites related to historic mining activities has recently
been reported. The purpose of our study is to contribute to existing tissue residue
guidelines, and focuses on aquatic oligochaetes that are major sediment
burrowers and deposit feeders, as a necessary tool in risk assessment studies.
Field metal bioaccumulation was measured in aquatic oligochaetes at several
unpolluted reference sites in the Nalón River basin to establish baseline values.
Metal (As, Cd, Cu, Cr, Hg, Ni, Pb, and Zn) tissue residues were measured
separately in indigenous microdriles and lumbricids (2014 survey) and compared
with Tubifex tubifex exposed to reference sediment in 28-d chronic bioassays
(2010-11 survey). Metal bioaccumulation values in reference sediments from
T. tubifex bioassays were generally similar, even when differences in sediment
metal concentration (mainly Hg and As) were notable. Metal tissue residues in
field vs bioassay worms exposed to Nalón River sediments only showed significant
differences for As and Pb. Although metal levels in oligochaetes from reference
sites are below threshold values reported for river macroinvertebrates, they
included the highest As levels [2.63-16.97 μg g-1 dw] and one of the highest Hg
levels (up to 0.62 μg g-1 dw) among a total of 11 macroinvertebrate taxa
examined in a risk assessment study of the Nalón River. Heavy metal (and
metalloid) baseline values for microdrile oligochaetes are needed to correctly
interpret deviations from the reference conditions in future sediment
bioassessment studies of this basin.
39
ABSTRACTS
ORAL
Phylogeography and geographic genetic analysis of Potamothrix
hammoniensis (Clitellata) which is a Ponto-Caspian origin having
distribution in some fresh water lakes of Turkey
Deniz Mercan (1), Naime Arslan (1) & Ertan Mahir Korkmaz (2)
(1) Department of Biology, Science and Art Faculty, Eskişehir Osmangazi
University, Eskisehir, Turkey; (2) Department of Molecular Biology and Genetics,
Science Faculty, Cumhuriyet University, Sivas, Turkey
Ponto-Caspian species among the Tubificidae, originating in the Black
Sea-Caspian Sea region, are continuously dispersing to the west over Central
Europe and to the north-west towards the Baltic Sea area and most of these
belong to genus of Potamothrix. Potamothrix hammoniensis is one of the
freshwater Clitellata species studied commonly. It is used almost 30 morphological
taxonomic characters for determination of P. hammoniensis becoming from
Annelids. It is predicted that this species can show speciation depends on
allopatric isolation as related to distribution barriers in phylogeographic history of
this species. It is aimed that presents distribution and phylogeography of
P. hammoniensis in some natural lakes of Turkey with molecular studies.
Oligochaeta samples were collected from Lakes of Gala, Büyük Akgöl, Sapanca,
Egirdir, Mogan, Cernek, Gölbaşi and Nemrut between 2010-2013. These samples
are belong to Potamothrix genus and look like P. hammoniensis as morphologically
but differ from some taxonomic characters which are primary rolling in
reproduction of P. hammoniensis determined until today at level of subspecies or
species so could not identify according to current identification keys. It is
identified both morphologically differentiations and analysis molecular data and
compared getting results. Fragments of COI and ITS2 were used in molecular
analysis. And it was examined network analysis and secondary structure of ITS2.
At the end of this research, it is determined whether this species is represented
with only one species within our country area or, samples determined in Gala
Lake may be new species, samples in Sapanca Lake and Egirdir Lake shown
differentiation, however this differentiation is not high to samples in Gala Lake.
Differentiation in Nemrut Lake samples also is high and it reach a conclusion that
these samples have different isolation process and mechanism from all studied
lakes samples.
40
ABSTRACTS
KEYNOTE LECTURE
Species specific morphological deformities in oligochaetes
associated with mercury emissions. Lake Vänern, Southern
Sweden, revisited
Göran Milbrink
Department of Ecology and Genetics, Animal Ecology, Uppsala University,
Uppsala, Sweden
The large oligotrophic Lake Vänern, southern Sweden, has been under the
influence from eutrophication culminating in the 1970s. Bottom fauna collections
from 1923 till the present day clearly demonstrate the processes of eutrophication
and thereafter oligotrophication of the lake. At the same time the lake has also
been heavily affected by industrial wastes containing heavy metals such as
mercury and other poisonous substances. Deformed chaetae of the oligochaete
species Potamothrix hammoniensis found in exposed bays of the lake in the late
1960s were described as a likely result of mercury in the environment. Fish were
thus locally banned from human consumption. Two decades later the most severe
chaetal abnormities are still there but have clearly decreased in occurrence.
Milder deformities, however, have not notably diminished. Two things have even
changed for the worse, i.e. also the oligotrophic indicator species Spirosperma
ferox is locally affected displaying both severe and mild deformities in anterior
ventral chaetal bundles. Deformities of both kinds are now also frequently
occurring in anterior dorsal bundles of P. hammoniensis. The combination of
mercury deposits in the sediments and low sedimentation rates are likely causing
agents.
41
ABSTRACTS
ORAL
Preliminary survey of freshwater Oligochaeta from selected
districts of Tamil Nadu.
Sivabalan S. Sampath & Mohammed Ibrahim Naveed*
Department of Zoology, The New College, Chennai - 600 014, India
A qualitative survey of fresh water Oligochaeta was conducted at Chennai,
Thiruvallur, Kanchipuram, Thiruvannamalai, Villupuram and Nilagiri districts of
Tamil Nadu from October 2013 to June 2015. Thirteen taxa were recorded from
a total of 1915 freshwater Oligochaeta examined from various random samples.
Out of the 13 taxa 10 were identified up to the species level, 2 were identified to
genus level and 1 up to family level. Aulophorus furcatus, Dero digitata, Dero
zeylanica (Naididae) and Pristina breviseta (Pristinidae) constitute the first report
for the Villupuram district. Bothrioneurum sp. (Tubificidae) constitutes the first
report for Nilagiri district. Dero digitata and D. zeylanica constitutes the first
report for Thiruvannamalai district. The Dero sp. recorded from Villupuram with 4
hair and 4 needle chaetae is uniquely different from the rest of the species
belonging to the genus Dero so far reported globally.
42
ABSTRACTS
ORAL
Oligochaetes (Annelida, Clitellata) in the Anzali International
Wetland, Northwestern Iran.
Fatemeh Nazarhaghighi (1), Tarmo Timm (2), Nader Shabanipour (3) & Rezvan
Mousavi Nadoushan (4)
(1) Department of Marine Biology, Faculty of Marine Sciences and Technology,
Tehran Sciences & Research Branch, Islamic Azad University, Tehran, Iran; (2)
Centre for Limnology, Estonian University of Life Sciences, 61117 Rannu,
Tartumaa, Estonia; (3) Department of Biology, Faculty of Science, University of
Guilan, Rasht, Iran; (4) Department of Marine Biology, Faculty of Marine Science
& Technology, Islamic Azad University, North Tehran Branch, Tehran, Iran
In order to determine the Oligochaeta fauna of the Anzali Wetland
(Northwestern Iran), samples of bottom sediment and aquatic vegetation were
collected from 13 stations 8 times from August 2012 to June 2013. As a result of
the study, 11 species and one genus were identified: Tubifex tubifex, Limnodrilus
claparedeianus, L. hoffmeisteri, Potamothrix hammoniensis, P. bedoti, Branchiura
sowerbyi, Nais pardalis, Ophidonais serpentina, Dero digitata, Stylaria lacustris,
Slavina appendiculata and Mesenchytraeus sp. Some physicochemical parameters
of the water (depth, temperature, dissolved oxygen and pH) were measured at the
sampling site. The average density of the total oligochaete community in the
benthos of the wetland was 6077 ind. m-2. Shannon-Wiener diversity index was
on average 0.92, with a maximum of 1.57 and a minimum of 0.44 at different
stations. Results indicated that the maximum and the minimum density were
observed in June (21837 ind. m-2) and in December (1041.9 ind. m-2)
respectively. Most frequent and abundant species were the tubificids
L. hoffmeisteri, L. claparedeianus, P. hammoniensis. All determined oligochaete
taxa except T. tubifex were new to the Anzali International Wetland, including
seven new to the fauna of Iran. Structure of the Oligochaeta community
correlated with progression of the trophic state of the aquatic ecosystem.
43
ABSTRACTS
ORAL
The first record of Branchiobdella kozarovi Subchev, 1978 from
Thrace Region-Turkey
Serpil Odabaşi (1), Naime Arslan (2) & Deniz Anil Odabaşi (1)
(1) Çanakkale Onsekiz Mart University, Marine Science and Technology Faculty,
Basic Sciences Department, Çanakkale, Turkey; (2) Eskişehir Osmangazi
University, Faculty of Arts and Science, Department of Biology, Meşelik, Eskişehir,
Turkey
In this study, the presence of the ectosymbiont Branchiobdella kozarovi
Subchev, 1978 on narrow-clawed crayfish, Astacus leptodactylus Eschscholtz,
1823, was observed first time in the Thrace Region of Turkey. The inspection
carried out on the crayfish obtained from Karpuzlu Reservoir (Ipsala-Edirne) in
January 2014 and we detected a total of 53 Branchiobdella kozarovi.
44
ABSTRACTS
ORAL
Diversity of Aulodrilus (Clitellata, Tubificinae) in East and Southeast
Asia
Akifumi Ohtaka
Department of Natural Science, Faculty of Education, Hirosaki University,
Hirosaki, Japan
The tubificine genus Aulodrilus is characterized by having a large number of
chaetae, short vasa deferentia and diffused sperms in spermathecae. About 10
valid species have so far been known in the world, and recent researches have
found several undescribed and some uncertain species from east and southeast
Asia. Anterior shift and duplication of the genital organs are found in many
species, and their segmental locations and numbers are often unstable even in a
single population. Southeast Asian species have male pores located in from III to
VII, being 4 to even 8 segments anterior than usual tubificine location, of which
those in VII is the most common. In temperate species, Aulodrilus japonicus and
A. americanus, only one segment or no anterior shifts were found in the location
of male pore. Duplication of the genital organ is found in the gonads, male ducts
and spermathecae. Aulodrilus species are generally thermophilous and they tend
to be abundant in shallow and eutrophic waters. Japanese A. japonicus and A.
limnobius become mature only in summer seasons. Along with Limnodrilus
hoffmeisteri and Branchiura sowerbyi, Aulodrilus species are common in rice
paddies and often become the most dominant oligochaetes comprising more than
80% of total oligochaete assemblages. In Japanese rice paddies, A. limnobius
surpassed others, while in southeast Asia, other congeners replaced, for example,
A. acutus and A. pigueti in Java, A. sp. 2 (undescribed) in Cambodia, and A. sp.
4 (undescribed) or A. cf. pectinatus in Myanmar. The dominant occurrence of
Aulodrilus species in rice paddies could be linked to their high abilities of asexual
reproduction and quick formation of tolerant forms against drying.
45
ABSTRACTS
ORAL
The role of Oligochaeta in the bioindication of stream
intermittency - results of the BIODROUGHT project
Petr Pařil (1,2), Vít Syrovátka (2), Michal Straka (3), Marek Polášek (1,2) &
Lenka Šikulová (2,3)
(1) T. G. Masaryk Water Research Institute, p. r. i., Prague, Czech Republic; (2)
Masaryk University Brno, Faculty of Science, Department of Botany and Zoology,
Brno, Czech Republic; (3) WELL Consulting Ltd., Brno, Czech Republic
The manifestations of climate change together with human alterations have
been affecting hydrological regime of streams progressively during the last
decades. The increase of stream intermittency brings new demands on water
management which is missing effective tools for the indication of drying up (i.e.
abruption of continual flow on a streambed surface) especially at small streams
up to 4th Strahler order. The developed method for the retrospective bioindication
of drought episodes in recent stream history provides a good chance to transfer
the results of scientific research into practice.
The ability of permanent fauna to reflect the "footprint of drought" depends on
local conditions of each site such as the availability of refugia (permeability of
hyporheic zone, duration of residual pools) and also on the recolonisation ability
and resistant stages of each macroinvertebrate taxa. Contrarily to many drought
sensitive groups (e.g. aquatic insects such as EPT taxa), the Oligochaeta are not
so dramatically depleted by drying up of a stream in terms of their abundance,
but rather their representation in the macroinvertebrate community is usually
higher immediately after re-flooding of the channel. On the other hand, the
Oligochaeta show remarkable turnover from typically aquatic specialists to
semiterrestrial taxa such as Enchytraeidae or Lumbdricids (e.g. Eiseniella
tetraedra) in the after-drought period and this lasting switch in the assemblage
composition increases the detectability of drought. The duration of dry episode
(from days to months) and the extent of dried stretch (from metres to kilometres,
with or without residuals pools) determine the magnitude of assemblage change
and enable to identify the degree of drought impact which plays an important role
in the complex assessment of stream vulnerability to drought.
The research in the BIODROUGHT project (www.biodrought.eu, grant
TA02020395) was supported by Technology agency of the Czech Republic.
46
ABSTRACTS
ORAL
Aquatic oligochaetes from southern Tibet, China
Yu Peng (1, 2), Hongzhu Wang (1) & Yongdee Cui (1)
(1) State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of
Hydrobiology, Chinese Academy of Sciences, Wuhan, China; (2) University of
Chinese Academy of Sciences, Beijing, China
The Tibetan Plateau is one of global biodiversity hotspots due to its unique
environments. Based upon investigations during 2006-2010, we have recorded
23 species of aquatic oligochaetes belonging to 2 families and 12 genera from
central Tibet, where altitude is 3,000-5,000 m a.s.l., annual precipitation is 300450 mm, and average temperature in the warmest month is 10-15°. Herein, we
report on preliminary findings of the aquatic oligochaetes fauna of southern Tibet,
based upon surveys during 2013-2015. Southern Tibet is the transitional zone
between the Tibet Plateau and the India Assam plain. Its environments are quite
heterogeneous, with altitude being <2,000 m-5,500 m a.s.l., annual precipitation being 200-4,000 mm, and average temperature in the warmest month being
10-25°. In the present paper, we discuss 38 species belonging to 3 families and
17 genera (Haplotaxidae: 1 genus and 1 species; Lumbriculidae: 1 genus and 2
species; Naididae: 15 genera and 35 species) from southern Tibet, including 1
new species, Tubifex dilata sp. n., and 3 new record species for China, Tubifex
montanus Kowalewski, 1919, Rhyacodrilus punctaus Hrabě, 1931 and Haplotaxis
glandularis (Yamaguchi, 1953). Among the family Naididae, 8 genera and 17
species belong to the subfamily Naidinae, 4 genera and 14 species to the
subfamily Tubificinae and 3 genera and 4 species to the subfamily Rhyacodrilinae.
The aquatic oligochaetes of southern Tibet are more species-rich than those of
central Tibet, and the known fauna has more Holarctic elements.
47
ABSTRACTS
KEYNOTE LECTURE
Sperm transfer by spermatophores in microdrile Oligochaetes
(Annelida, Clitellata)
PIlar Rodriguez (1) & Steven V. Fend (2)
(1) Department of Zoology and Animal Cell Biology, Faculty of Science and
Technology, University of the Basque Country, Box 644, 48080 Bilbao, Spain; (2)
U.S. Geological Survey, 345 Middlefield Rd., Menlo Park CA 94025, USA
The formation of encapsulated spermatophores is exceptional among aquatic
oligochaetes, although it seems to have occurred independently in several
unrelated taxa. Among the microdriles, some variations appear unique to single
species. The recently-described lumbriculid Uktena riparia (Fend et al.) forms
spermatophores in the male duct and attaches them within a deep spermathecal
bursa, a character unique in microdriles. The attachment of spermatophores to
other specific copulatory areas, as in acanthobdellid and piscicolid leeches, has
been reported only in Paranadrilus Gavrilov. Anatomical comparison of Uktena,
Bothrioneurum Stolc, Paranadrilus, and Aktedrilus Knöllner suggests convergence
of glandular organs in the male duct used for the transfer and/or attachment of
spermatophores to the concopulant worms. The presence of similar organs in
other microdrile taxa (e.g. Smithsonidrilus Brinkhurst) where spermatophores have
not been reported is discussed.
48
ABSTRACTS
ORAL
Can oligochaete assemblages predict an ecological type of treeless
spring fens?
Jana Schenková, Martina Bílková & Michal Horsák
Department of Botany and Zoology, Masaryk University, Brno, Czech Republic
The study was located in the Western Carpathian Mountains in the Czech and
Slovak Republics. Fifty-four permanent treeless spring fens fed by groundwater
were chosen for the study to cover a gradient of groundwater chemistry. The study
sites can be classified into the four main types based on water chemistry and
vegetation: (i) extremely mineral-rich fens with a tufa formation, (ii) brown-moss
mineral-rich fens without the tufa, (iii) mineral-rich Sphagnum fens with the
occurrence of calcitolerant Sphagnum species, and (iv) mineral-poor Sphagnum
fens. From 2006 to 2012, samples of aquatic macroinvertebrates were collected
in spring and autumn at each study site from three contrasting mesohabitats: two
patches in the spring area with flowing and standing water and spring brook.
Using Principal Coordinate Analysis (PCoA) we observed two main gradients of
species composition variance, which covered 31.1% and 16.0% of the entire
variation, respectively. The main compositional change (1st PCoA axis), was
related mainly to the variation of total organic carbon (TOC) in substratum and
partly also Ca+Mg concentration, the second main direction (2nd PCoA axis) was
associated mainly with the amount of organic matter and water temperature.
Four main spring fen types determined by composition of vegetation were
identified also by clitellate species. We found several species with a significant
association with all four mineral types of fens, with more species preferring
significantly both types of Sphagnum fens in contrast to the remaining types of
alkaline fens. However, most of these indicator species showed only a high
probability to be found within the group, but rather lower specificity. In contrast,
some species with a high specificity were very rare with a low frequency and
abundance. Our study also showed that pristine and still well-preserved spring
fens habitats can be easily destroyed by organic matter input. Supported by
P505/11/0779, GA15-15548S, MUNI/A/0888/2013.
49
ABSTRACTS
ORAL
Annelids of six calcareous spring fens and adjacent grasslands in
the Western Carpathians
Jiří Schlaghamerský (1), Jana Schenková (1), Martina Bílková (1) & Václav Pižl
(2)
(1) Department of Botany and Zoology, Faculty of Science, Masaryk University,
Brno, Czech Republic; (2) Institute of Soil Biology, Biology Centre of the Czech
Academy of Sciences, České Budějovice, Czech Republic
Although wetlands have received substantial attention by researchers,
knowledge on the invertebrates of the transition zone between aquatic sediments
and terrestrial soils remains poor. The present results are the first obtained
within a larger project that attempts (amongst others) to bridge this gap. The
object of interest were assemblages of annelids, represented by several families
and of ecological importance in both types of habitat. Six calcareous spring fens
(pH 8.5-8.9) and adjacent meadows or pastures were sampled on April 27-28,
2015. The sites were located in the hills and mountains (450-750 m a.s.l.) of the
Western Carpathians in eastern Moravia (Czechia) and western Slovakia.
Microannelids were sampled with a cylindrical corer of 17 cm2 working area to
10-15 cm depth. Five cores were taken in the fen and five in the grassland per
site. Megadriles (potentially also leeches) were sampled using a cylindrical corer
of 625 cm2 working area to 20 cm depth. At each site two cores in the fen, two in
the grassland and two at their transition were taken. In the drier parts of the
gradient earthworms were extracted from underlying soil by diluted formaldehyde
poured into the holes after core removal. Annelids from the soil cores were
extracted in the lab (wet funnel extraction for microannelids, heat extraction in a
Kempson apparatus for megadriles). Microannelids were identified alive to allow
for enchytraeid identification to species, earthworms fixed in formaldehyde. In
total, 919 microannelids were obtained, belonging to 41 species of
Aeolosomatidae, Enchytraeidae, Lumbriculidae, and Naididae (incl. former
Tubificidae). The large cores yielded 198 earthworms (Lumbricidae) of 12 species.
Eiseniella tetraedra was dominant in the fen samples, while the hygrophilous
Octolasion tyrtaeum and Octodrilus transpadanus were found in few specimens
mostly in the transition zone. Annelid assemblages differed markedly both
between fens and grasslands and between sites. Supported by GA15-15548S.
50
ABSTRACTS
ORAL
Enchytraeids from first order streams in Sao Paulo State, Brazil
Rüdiger M. Schmelz (1,3), Roberto da Gama Alves (2) & Rut Collado de la Pena
(3)
(1) ECT Oekotoxikologie GmbH, Böttgerstr. 2-14, 65439 Flörsheim, Germany; (2)
Laboratório de Invertebrados Bentônicos, Departamento de Zoologia, Instituto de
Ciencias Biológicas, Universidade Federal de Juiz de Fora (UFJF), Campus
Universitário, s/n, CEP 36036 900, Juiz de Fora, MG, Brasil; (3) Universidad de A
~
~
Corun
a, Fac. Ciencias, Biología Animal, Rua da Fraga, 10, 15008 A Corun
a,
Spain
Aquatic oligochaetes were collected in first order streams in the "Parque
Estadual Campos do Jordao", Sao Paulo State, Brazil, during several sampling
campaigns in 2006 to 2008. Work was carried out in the framework of the
project BIOTA/FAPESP, "Research Program on Biodiversity Characterization,
Conservation, Restoration and Sustainable Use" (www.biota.org.br). A small
percentage of the collected material consisted of enchytraeids. Eight species taxa
were distinguished, and three of them could be named. Almost half of the
specimens belonged to Achaeta singularis Schmelz, 2008, described from sites in
the Mata Atlântica in Paraná, some 250 km southwest. Presence of adult
specimens allowed to complete the redescription of that species, which belongs to
the plesiomorph sub-group with lateral spermathecal pores and no pyriform
glands. All adult or subadult specimens had the gonadal region shifted two
segments anteriad. The curious crystals in the coelom, originally described and
present in this material, may consist of oxal acetate. A single specimen agreed in
all observable details with Marionina deminuta Rota, 2013. Four taxa of
Guaranidrilus were distinguished but not named. The high percentage of Achaeta
singularis in the collection suggests that the species' natural habiat is in or close
to the rivers. Other species may be accidental finds from the adjacent terrestrial
enchytraeid fauna.
51
ABSTRACTS
ORAL
Phreodrilidae in Irish peatlands
Rüdiger M. Schmelz (1,2), Rut Collado (2), Rachel Wisdom (3) & Tom Bolger (3)
(1) ECT Oekotoxikologie GmbH, Böttgerstr. 2-14, 65439 Flörsheim, Germany; (2)
~
Universidad de A Corun
a, Fac. Ciencias, Biología Animal, Rua da Fraga, 10,
~
15008 A Coruna, Spain; (3) School of Biology and Environmental Science,
University College Dublin, Belfield, Dublin 4, Republic of Ireland
A strange inhabitant of Irish boglands was discovered in Co. Mayo in 2011, a
microdrile oligochaete worm of the family Phreodrilidae. This family has a marked
southern distribution, so the find of phreodrilids in Ireland is somewhat surprising,
although this is not the first find of a representative of that family in Ireland. The
specimens occurred at 3 of the 12 sites sampled, and more than 100 specimens
were collected, which suggests a stable population rather than an accidental
'visitor'. Specimens were minute, not longer than 3-4 mm when alive (1-2 mm
after fixation), and they belong to a hitherto unknown species of the genus
Insulodrilus. The new species is conspicuous by prominent epidermal gland cells
that cover all sides of the body in anterior segments but are restricted to the
dorsal side in posterior segments, here forming thick segmental cushions,
conspicuous in living and preserved material. There are droplet-like structures
beneath the body wall, associated with chaetal retractor muscles. Other
characters are common in the family. The phreodrilids co-occurred with
enchytraeid species that are indicators of wetness and acidity, which sheds some
light on the ecology of this family. The curious find raises the question whether
this species is introduced or part of the natural fauna of Ireland.
52
ABSTRACTS
ORAL
Structure and evolution of ovaries in oligochaetous annelids:
recent progress and things to do
Piotr Świątek
Department of Animal Histology and Embryology, University of Silesia, Katowice,
Poland
The classical descriptions of the ovary structure and oogenesis in Oligochaeta
have taken into account: the ovary shape (e.g., small, conically shaped ovaries in
lumbriculids and tubificine naidids or grape-like ovaries in enchytraeids) and/or
‘solitary’ (the development of oocytes occurs within ovaries – lumbriculids,
tubificine Naididae, lumbricids and other megadriles) and ‘nutritional’ (the
original ovary splits into cell groups that float in the egg sac or coelom – Naidinae,
enchytraeids and phreodrilids) modality of oogenesis. However, ultrastructural
studies have revealed, that no matter what the ovary shape is, germ-line cells
always form syncytial group of cells (i.e., cysts). The characteristic feature of these
cysts is the presence of a cytophore, i.e., an anuclear cytoplasmic mass in the
center of the cyst to which all germ cells are connected. Morphological
observations suggest that two groups of cells differentiate within the cysts –
oocytes and nurse cells, and therefore the ovaries of Oligochaeta are meroistic
(nutritional). The only exception has recently been found in Capilloventer australis
(Capilloventridae), in which no cysts are formed and oogenesis seems to be
panoistic (solitary). Although in recent years the ovary organization and
oogenesis in Oligochaeta have been intensively studied (e.g., lumbricids,
lumbriculids, tubificine naidids, propappids), the ways in which ovaries evolve is
far from being elucidated. The detailed ovary organization is still unknown in
some important groups such as haplotaxids or phreodrilids, whereas in some
groups (e.g., enchytraeids) the ovary needs to be redescribed. Similarly, the
ovaries in these polychaetous annelids, which are regarded as potential sister
groups for Clitellata, also need more detailed studies. At present, only a
preliminary scenario of ovary evolution in Oligocheata can be presented. This
work was partially funded by Polish National Science Centre. Contract grant
number: DEC-2012/05/B/NZ4/0241
53
ABSTRACTS
KEYNOTE LECTURE
Fate of Lamprodrilus isoporus Michaelsen, 1901 (Oligochaeta:
Lumbriculidae) in eutrophic lakes
Tarmo Timm
Estonian University of Life Sciences, Centre for Limnology, Rannu, Tartumaa,
Estonia
Most species of the genus Lamprodrilus (19 of total 24) inhabit Lake Baikal, 16
of them being endemic for this lake. The distribution area of L. isoporus has split
up, with one half in Baikal and connected with it lakes, and the other in Northern
Europe (Karelia, Finland, Central Sweden, North-Western Russia, and Estonia).
The European population was originally described as L. isoporus f. variabilis
Svetlov, 1936; however, it does not differ from the Siberian representatives except
for their twice as large size in Lake Baikal only. The typical habitat of L. isoporus
is the cool and oxygen-rich profundal of oligotrophic lakes. However, in Lake
Baikal it avoids the deepest zones, while in Lake Onega (Karelia) it flourishes also
in the muddy and sandy littoral and does not avoid weakly polluted areas. In the
~
Estonian shallow eutrophic lakes of Peipsi and Vo
rtsjärv, L. isoporus cannot
survive in the profundal because of high summer temperatures and oxygen
deficiency. Instead, it is widely distributed in the unvegetated, wave-washed sandy
littoral and sublittoral of Lake Peipsi at depths of 1-7 m. In very shallow and more
~
rtsjärv, its habitat is limited to depths of 0.5-1 m in the leeward
eutrophic Lake Vo
corner of the lake. L. isoporus reproduces only in a sexual way and in these two
lakes only in winter, under the ice cover. In the summer months, its population
consists of two cohorts, both sexually immature: the older individuals with the
reproductive system resorbed after egg-laying and the smaller young worms. Both
cohorts begin to mature in autumn when the water is cooling down. L. isoporus
was very abundant in Lake Peipsi from the 1960s up to the 1980s, accounting for
up to one fourth or one third of Oligochaeta in the quantitative zoobenthos
samples. Its frequency and abundance decreased drastically beginning from the
1990s, about an order of magnitude by 2010. The reason can lie in the
synergistic impact of progressive eutrophication (water blooms of toxic
cyanobacteria, expansion of reeds) and the introduction of an invasive gammarid,
~
Gmelinoides fasciatus. In Lake Vo
rtsjärv L. isoporus was still widely distributed in
1959 but was limited to a single station in the subsequent years; it has not been
recorded there after 1988. L. isoporus can be ecologically compared to the
"glacial relict" crustaceans but, as it is lacking in the large Swedish lakes as well
as in many other oligotrophic lakes of Northern Europe and Siberia, may have a
different distribution history. The present-day bipartite distribution area is
certainly a relict of the former broader continuous area that probably split up
long before the last glaciation. The question about whether L. isoporus originates
from Lake Baikal, or has been close to an Euro-Siberian species that once
invaded Lake Baikal and has possibly become an ancestor of the numerous
Lamprodrilus spp. in this lake remains still unclear.
54
ABSTRACTS
POSTER
Ovary composition and oogenesis in the sludge worm Tubifex
tubifex (Tubificinae).
Anna Z. Urbisz, Łukasz Chajec & Piotr Świątek
Department of Animal Histology and Embryology, University of Silesia, Katowice,
Poland
The paired ovaries in Tubifex tubifex are small (~2mm long) and polarized
structures. Their narrow ends are connected to the intersegmental septum via
ligaments. Within each ovary the gradient of germ cell development occurs along
the long ovary axis and three zones can be distinguished – zone I, which includes
the oogonia, zone II with undifferentiated meiotic germ cells and zone III in which
the germ cells differentiate into two morphologically distinct categories – nurse
cells and oocytes. During oogenesis, oocytes continue meiosis and grow
considerably gathering numerous organelles and storage material. The growing
oocytes are situated linearly on the one side of the ovary surface. Early
vitellogenic oocytes detach from the ovary and flow freely in the coelomic fluid.
Somatic cells accompany the germ cells both inside and around the ovary and
form a thin outer ovary envelope. The entire ovary of T. tubifex is composed of
only one germ-line cyst. The average number of germ cells is 2,000 with eight
oocytes growing at a time on average. The formation of germ-line cysts is a
conserved phase of gametogenesis in both most invertebrate and vertebrate
animals. The organization of germ-line cysts varies between different taxa.
However in T. tubifex, as in other clitellate annelids that have been studied to
date, the architecture of the cysts is broadly similar, i.e. the center of the cyst is
occupied by a common, anuclear cytoplasmic mass (cytophore) and the germ cells
are localized on its periphery. Each germ cell is connected to the cytophore via
one stable intercellular bridge. The cytophore in the T. tubifex cyst is long and
branched and it reaches its maximal dimension in zone III. Well-developed
cytoskeletal elements, mainly in the form of prominent strands of actin filaments
inside the cytophore and the ring canal wall, occur in the germ-line cyst of T.
tubifex. It appears that the microfilaments ensure the integrity of such a
multicellular cyst.
55
ABSTRACTS
ORAL
Annelidically speaking – 2015
Mark J. Wetzel
Illinois Natural History Survey, Prairie Research Institute at the University of Illinois
at Urbana-Champaign, Champaign, IL 61820 USA
This presentation will highlight: 1) Nomenclatura Oligochaetologica Editio
Secunda – a web-based global catalogue of names, descriptions, and type
specimens of the Oligochaeta; 2) the status of semi-aquatic, limicolous, and
terrestrial oligochaetes in North America; 3) distributional records for several
aquatic oligochaetes considered introductions to North American waters; 4)
freshwater oligochaetes associated with natural and anthropogenic phytotelmata
in the Florida Keys; 5) the Society for Freshwater Science Taxonomic Certification
Program and the digital image-based test for freshwater oligochaetes; 6) a
timeline for a new guide with keys to aquatic oligochaetes of North America, and
7) a collections profiling exercise, version 3.
56
ABSTRACTS
ORAL
An updated phylogeny of the marine enchytraeid genus Grania
supports cryptic speciation and geographic structuring
Pierre de Wit (1), Allesandro Livio Prantoni (2) & Christer Erséus (3)
(1) University of Gothenburg, Deptartment of Marine Science, Tjärnö. SE-45296
Strömstad, Sweden; (2) Federal University of Paraná State, Center for Marine
Studies, Av. Beira Mar, s/n, zip code 83255-976, Pontal do Paraná, Paraná, Brazil;
(3) University of Gothenburg, Dept. of Biological and Environmental Sciences, Box
463, SE 405 30 Göteborg, Sweden
Grania is an enchytraeid genus with a world-wide marine distribution in sandy
sediments. Previous genetic work has indicated an evolutionary pattern matching
geographic species distributions, suggesting low dispersal capabilities. At the
same time, a recently described cryptic species within the genus have called into
question the previously described morphological diversity of Grania. However,
sampling efforts have to date been concentrated on Europe, North America and
Australia, making it difficult to draw general conclusions about the evolutionary
history of the genus. Here, we use the first-ever genetic data from Grania species
from South America (Chile and Brasil) and South Africa in combination with
previously published data in order to produce an updated multi-locus molecular
phylogeny of the genus. The previously seen geographic substructuring of the
genus is further supported by the new data, as is the previously described cryptic
species Grania occulta. We also find a new example of cryptic speciation in South
Africa, and within-species population sub-structuring along a geographic cline on
the Chilean coast, further supporting the lack of dispersal capability in this genus
and the resulting evolution of cryptic diversity.
57
LIST OF PARTICIPANTS
Achurra Ainara
University of The Basque Country
UPV/EHU, Sarriena s/n, 48940 Leioa,
Spain
ainara.achurra[at]ehu.es
Erséus Christer
Department of Biological and
Environmental Sciences,
Box 463 SE-40530, Göteborg, Sweden
christer.erseus[at]bioenv.gu.se
Aras Seval
Nevsehir Haci Bektaş Veli University,
Faculty
of
Engineering
and
Architecture,
Department
of
Environmental Engineering, Nevsehir,
Turkey
sevalkokmen[at]gmail.com
Fend Steven
17650 Kilkenny Road, 95030 Los
Gatos, California, USA
stevenfend[at]gmail.com
Arslan Naime
Eskisehir Osmangazi
Eskisehir, Turkey
oligo2009[at]gmail.com
University,
Bílková Martina
Masaryk University, Faculty of
Science, Department of Botany and
Zoology, Kotlářská 2, 61137 Brno,
Czech Republic
maty.bilkova[at]seznam.cz
Cui Yongde
Institute of Hydrobiology, Chinese
Academy of Sciences, No. 7 Donghu
South Road, 430072 Wuhan, China
ydcui[at]ihb.ac.cn
Dumnicka Elzbieta
Institute of Nature Conservation, PAS
al. A. Mickiewicza 33, 31-120,
Krakow, Poland
dumnicka[at]iop.krakow.pl
Eriksson Ma° rten
Department of Biological and
Environmental Sciences, Göteborg,
Sweden
gusmarter[at]student.gu.se
58
Findik Ozlem
Nevsehir Haci Bektas Veli Unv. Arts
and Sciences Faculty, Moleculer
Biology and Genetics Department,
Nevsehir, Turkey
ofindik74[at]yahoo.com
Gelder Stuart
University of Marine at Presgue Isle,
13 Merganser St., Westbrook, Maine
04092, USA
stuart.gelder[at]umpi.edu
Giere Olav
Univ. of Hamburg, Zool. Institute,
Hamburg, Germany
olav.giere[at]uni-hamburg.de
Gorgoń Szymon
University of Silesia, Department of
Animal Histology and Embryology
Bankowa 12, 40-007 Katowice,
Poland
szymon.gorgon88[at]gmail.com
van Haaren Ton
Grontmij / team ecology, Sciencepark
406
1098
XH,
Amsterdam,
Netherlands
ton.vanhaaren[at]grontmij.nl
Hirabayashi Kimio
Department of Applied Biology,
Shinshu University 3-15-1, Tokida
386-8567 Ueda, Nagano Prefecture,
Japan
kimio[at]shinshu-u.ac.jp
Lee Jeounghee
The Division of EcoCreative Ewha
Womans University, 52, Ewhayeodaegil, Seodaemun-gu, 120-750 Seoul,
Korea
tinysky1004[at]gmail.com
Ito Katsutoshi
National Research Institute of
Fisheries and Environment of Inland
Sea, Fisheries Research Agency
Maruishi 2-17-5 739-0452
Hatsukaichi, Hiroshima, Japan
katsuit[at]affrc.go.jp
Liu Yingkui
Goteborgs Universitet medecinaregatan 18A, Gothenburg, Sweden
403382944[at]qq.com
Ito Mana
National Research Institute of
Fisheries and Environment of Inland
Sea, Fisheries Research Agency
Maruishi
2-17-5
739-0452
Hatsukaichi, Hiroshima, Japan
nozakim[at]affrc.go.jp
Jaweir Jawad Jabir, Haifa
University of Baghdad, Al-Jaderyia Baghdad, Iraq
hjaweir[at]yahoo.com
Jelinek Herbert
Centrum fur Naturkunde, MartinLuther-King-Platz 3, 20146 Hamburg,
Germany
hjelinek[at]gmx.de
Kaygorodova Irina
Limnological Institute, 3 UlanBatorskaya st., 664033 Irkutsk, Russia
irina[at]lin.irk.ru
Krodkiewska Mariola
University of Silesia, Bankowa 9
40-007 Katowice, Poland
mariola.krodkiewska[at]us.edu.pl
Martin Patrick
Royal Belgian Institute of Natural
Sciences 29 rue Vautier, 1000
Brussels, Belgium
patrick.martin[at]naturalsciences.be
Martinsson Svante
University of Gothenburg, Göteborg,
Sweden
svante.martinsson[at]gu.se
Méndez-Fernández Leire
University of the Basque Country
(UPV/EHU), Sarriena S/N, 48940
Leioa, Spain
leire.mendez[at]ehu.eus
Mercan Deniz
Eskisehir Osmangazi University,
Turkey
deniss-kara[at]hotmail.com
Milbrink Göran
Department of Ecology and Genetics,
Animal Ecology, Uppsala University
Norbyvägen 18D, 75236 Uppsala,
Sweden
goran.milbrink[at]ebc.uu.se
Naveed Mohammed Ibrahim
P.G & Research Department of
Zoology, The New College
Chennai - 600 014, India
naveed_newcollege[at]rediffmail.com
59
Nazarhaghighi Fatemeh
Department of Marine Biology,
Faculty of Marine Sciences and
Technology, Tehran Sciences &
Research Branch, Islamic Azad
University, Tehran, Iran
hiva582005[at]yahoo.com
Ohtaka Akifumi
Hirosaki University, Bunkyo-cho 1
036-8560 Hirosaki, Japan
ohtaka[at]hirosaki-u.ac.jp
Pařil Petr
T. G. Masaryk water research institute
p.r.i., Podbabská 30, 16000, Prague,
Czech Republic
paril[at]sci.muni.cz
Peng Yu
Institute of Hydrobiology, Chinese
Academy of Sciences, No. 7 Donghu
South Road, 430072 Wuhan, China
pydaisy[at]163.com
Rodriguez Pilar
University of Basque Country
Box 644 48080, Bilbao, Spain
pilar.rodriguez[at]ehu.eus
Schenková Jana
Czech Republic
schenk[at]sci.muni.cz
Schlaghamerský Jiří
Czech Republic
jiris[at]sci.muni.cz
60
Soors Jan
INBO, Kliniekstraat 25, 1070 Brussel,
Belgium
jan.soors[at]inbo.be
Świątek Piotr
University of Silesia
Bankowa 12, 40007 Katowice, Poland
piotr.swiatek[at]us.edu.pl
Timm Tarmo
Estonian University of Life Sciences,
Centre for Limnology, Limnologia tee
2/1-4, EE61117, Rannu, Tartumaa,
Estonia
tarmo.timm[at]emu.ee
Urbisz Zofia Anna
University of Silesia, Department of
Animal Histology and Embryology
Bankowa 12, 40-007 Katowice, Poland
anna.urbisz[at]us.edu.pl
Wetzel J. Mark
Research Scientist Illinois, Natural
History Survey, Prairie Research
Institute at the University of Illinois at
Urbana-Champaign, 1816 S. Oak
Street, MC-652 61820 Champaign,
Illinois, USA
mjwetzel[at]illinois.edu
Wit de Pierre
University of Gothenburg
Hättebäcksvägen 7, 45296
Strömstad, Sweden
pierre.de_wit[at]bioenv.gu.se
Wuillot Jean
Iris consultants, France
irisconsu[at]wanadoo.fr
Schmelz M. Rüdiger
ECT
Oekotoxikologie
GmbH,
Böttgerstr. 2-15, 65439 Flörsheim,
Germany
rmschmelz[at]gmail.com
AUTHOR'S INDEX
AUTHOR'S INDEX
A
Achurra Ainara............................ 15
Al-Sarai M. H.............................. 30
Alves da Gama Roberto............... 51
Aras Seval..............................16, 24
Arslan Naime...................17, 40, 44
B
Bolger Tom.................................. 52
Barişik Burcu............................... 17
Bílková Martina................18, 49, 50
C
Carter L. James............................23
des Châtelliers Creuzé Michel...... 36
Chajec Łukasz..............................55
Cui Yong-De.......................... 19, 47
D
Dumnicka Elzbieta....................... 20
E
Eriksson Marten........................... 21
Erséus Christer...........15, 21, 22, 35
F
Fend V. Steven................. 15, 23, 48
Findik Özlem......................... 16, 24
G
Giacomazzi Federica................... 37
Giere Olav.................................. 25
Gorgoń Szymon.......................... 26
H
Hano Takeshi........................ 28, 29
Hellmann Nadja.......................... 25
Hirabayashi Kimio....................... 27
Horsák Michal............................. 49
Höger Ulrich............................... 25
I
Ito Katsutoshi........................ 28, 29
Ito Kazuki.................................... 29
Ito Mana............................... 28, 29
J
Jaweir Jawab Jabir Haifa............. 30
Jung Jongwoo............................. 34
K
Kalyoncu Hasan........................... 17
Kaygorodova A. Irina............ 31, 32
Kostecki Maciej............................ 33
Krodkiewska Mariola............. 26, 33
Korkmaz Mahir Ertan................... 40
L
Lee Jeounghee............................ 34
Liu Yingkui.................................. 35
M
Małota Karol............................... 26
Marchant Richard........................ 26
Martin Patrick.............................. 36
Martínez-Ansemil Enrique............ 37
Martínez-Madrid Maite................ 39
Martinsson Svante................. 21, 38
Méndez-Fernández Leire..............39
Mercan Deniz........................ 17, 40
Milbrink Göran............................ 41
Mochida Kazuhiko................. 28, 29
N
Nadoushan Mousavi Rezvan........ 43
Naveed Ibrahim Mohammed....... 42
Nazarhaghighi Fatemeh.............. 43
61
AUTHOR'S INDEX
O
Odabaşi Serpil............................. 44
Odabaşi Anil Deniz......................44
Ohkubo Nobuyuki................. 28, 29
Ohtaka Akifumi..................... 27, 45
Ohta Kohei........................... 28, 29
Olivier Marie-José....................... 36
Onduka Toshimitsu................ 28, 29
W
Wang Hongzhu................... 19, 47
Wirkner S. Christia ..................... 25
Wisdom Rachel............................ 52
de Wit Pierre............................... 57
Wetzel J. Mark............................. 56
Z
Zhao Weihua.............................. 19
P
Pařil Petr...................................... 46
Pena Collado de la Rut.......... 51, 52
Peng Yu....................................... 47
Pižl Václav................................... 50
Płachno J. Bartosz........................ 26
Polášek Marek............................. 46
R
Rodriguez Pilar.................15, 39, 48
S
Salur Ali.......................................17
Sambugar Beatrice.......................37
Sampath S. Sivabalan.................. 42
Shabanipour Nader..................... 43
Schenková Jana...............18, 49, 50
Schlaghamerský Jiří..................... 50
Schmelz M. Rüdiger......... 36, 51, 52
Scholz Stephan............................ 25
Stauffer-Olsen Natalie................. 23
Steinmann David......................... 25
Straka Michal.............................. 46
Syrovátka Vít............................... 46
Świątek Piotr.................... 26, 53, 55
Š
Šikulová Lenka............................ 46
T
Timm Tarmo.......................... 43, 54
U
Urbisz Z. Anna...................... 26, 55
62
SPONSORS
63
SPONSORS
64
SPONSORS
Czech Zoological Society was founded as the Czechoslovak Zoological Society in
Prague on March 7th, 1927, having been initiated by several university professors,
viz. František Vejdovský, Jaromír Wenig, Karel Šulc and Jan Zavřel. The first
committee included 19 members with Professor F. Vejdovský as the President. Since
then, the Society has brought together specialists dealing with all branches of
zoology, including invertebrates and vertebrates. The purpose was to contribute to
the development of zoology and all its branches, organize lectures and excursions, to
convene congresses, and to publish scientific articles and thus contribute to the
development of zoology. During its existence, the name of the Society has been
changed several times:
Czechoslovak Zoological Society (1927-1939)
Czech Zoological Society (1939-1945)
Czechoslovak Zoological Society (1945-1952)
Czechoslovak Zoological Society attached to the Czechoslovak Academy of
Sciences (1952-1992)
Czech Zoological Society (since 1993, after the dissolution of
Czechoslovakia).
The society currently publishes the international journal Acta Societatis Zoologicae
Bohemicae (in English), two small periodicals, i. e. Informační zpravodaj and
Zprávy České zoologické společnosti (both in Czech) and the proceedings of various
conferences and seminars. The journals in the library of the Czech Zoological
Society (ca 21,000 volumes) were obtained by exchange with numerous scientific
societies and institutions and currently it includes a unique collection of 902
periodicals mainly of foreign origin. At present, the Czech Zoological Society has
231 members.
Sergej Hrabě (1899-1984), excellent zoologist, specialized in the study of aquatic
worms (Oligochaeta), professor at the University in Brno, was the member of the
society from its founding in 1927 until his death in 1984.
More information: www.zoospol.cz
65
NOTES
66
NOTES
67
NOTES
68

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