observation of platn tropism

Students: Jiří Kyslík, Jan Novotný, Martin Šíp
Supervisor: Ing. Vítězslav Březina, CSc.
This project has received financal support from the Europian Social Fund
and from goverment of the Czech Republic
Project objectives
experimental studies of tropical
movements plants under the influence
of phytohormones
 assessment of motion, statistical
analysis, scientific work, time-lapse
cinema
 proof of the theoretical model

Tropisms
oriented movements of plants affected
by external stimulus, non-locomotion
 classification
– gravitropism
– phototropism
– thigmotropism
– chemotropism
– hydrotropism

Phytohormones
plant growth regulators
– stimulators (auxin), inhibitors (abcis a.)
 auxin – IAA
– synthesized in the top plants, transport
– regulation of growth, influenced by
external factors
– auxin as a signal substance causes a
tropism
– biosynthesis

Methods
time-lapse cinematography, analysis
– illumination, spectral analysis
 gadget
– biomodel: Avena sativa
– camera: Nikon D90
– software: ImagetoAVI 1.0.0.5, ImageJ
1.4.3.67

Direct ligthing
Direct lighting
650
R² = 0,9944
600
Lenght [ppi]
550
500
450
400
350
300
250
0:00
1:12
2:24
3:36
4:48
Time [min]
6:00
7:12
8:24
Side lighting
Side lighting
30
R² = 0,9845
20
Turn [angle]
10
0
0:00
-10
0:28
0:57
-20
-30
-40
Time [min]
1:26
1:55
Red light
Blue light
Methods
time-lapse cinematography, analysis
 Gadget
– camera: moticam 2000
– biomodel: Pisum sativum convar.
medullare
– software: ImagetoAVI 1.0.0.5, ImageJ
1.4.3.67

Growth, distilled water
Growth, distilled water
1,9
1,7
Turn [rad]
1,5
R² = 0,661
1,3
1,1
0,9
0,7
0,5
0,3
0:00
2:24
4:48
7:12
9:36
Time [min]
12:00
14:24
16:48
19:12
Growth, water
Growth, water
3
2,5
Turn [rad]
2
1,5
R² = 0,880
1
0,5
0
0:00
2:24
4:48
7:12
Time [min]
9:36
12:00
Root response to auxin

auxin (IAA) mixed with agar (BBM)
– concentration of auxin – 0.1 M
– concentration of auxin – 0.01 M
– concentration of auxin – 0.000001 M
Auxin, 10-1 M
Auxin, 10-2 M
Auxin, 10-6 M
Conclusion
proving that the vital reaction and
analysis is critical alert
 experimental verification of the
theoretical model
 mastery of methods and time-lapse
cinematography

Resources
Regulátory rostlinného růstu – S.
Procházka, J. Šebánek a kol., 1997; ISBN
80-200-0597-8
 Fyziologie rostlin – S. Procházka, I.
Macháčková, J. Krekule, J. Šebánek a
kol., 1998; ISBN 80-200-0586-2
 http://cs.wikipedia.org/wiki/Auxin

Acknowledgment
Supervisor: Ing. Vítězslav Březina, CSc.
 Technical support: pan Jan
Novák, Ing. Štěpánka Kučerová, paní
Ilona Slepičková

This project has received financal support from the Europian Social Fund
and from goverment of the Czech Republic